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- W1972194009 abstract "Attractive economics and short development timelines have often been cited as reasons for using bacteria to express eukaryotic proteins on a commercial scale. Nevertheless, routine techniques for bacterial expression of heterologous proteins are beset by a variety of technical and legal difficulties. In particular, the use of plasmids to express foreign proteins, popular promoter systems, protein fusion partners, and histidine tags and the recovery of proteins from inclusion bodies are affected by a host of issued patents. Chromosomally encoded leaderless fusions (CELF) offer a variety of technical and legal advantages over existing bacterial expression systems. In this study, we show that CELF can be used to produce a wide assortment of eukaryotic proteins at 10-liter fermentation scale." @default.
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- W1972194009 date "1998-11-01" @default.
- W1972194009 modified "2023-09-27" @default.
- W1972194009 title "High-Level Expression of Eukaryotic Polypeptides from Bacterial Chromosomes" @default.
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- W1972194009 doi "https://doi.org/10.1006/prep.1998.0973" @default.
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