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- W1972238930 abstract "The proximal region of the γ-zein promoter (γ Z ) has a functional bifactorial prolamin box element containing two cis -acting elements, a prolamin-box motif (Pb3) and a GCN4-like motif (GZM). By particle bombardment of maize endosperms with 5′ deletions and internal deletions of γ Z fused to the GUS gene, we have shown that a 135 bp region containing the bifactorial element is involved in the transcriptional activation of the γ Z promoter. However, the 135 bp region was unable to activate the γ Z promoter in the absence of a 84 bp downstream sequence. Using in vivo footprinting and gel mobility shift assays with 15 DAP endosperm nuclear extracts, we have demonstrated the presence of trans -acting factors that interact with Pb3 and GZM target sites. Base-substitution mutations within Pb3 and GZM decreased transcription activity of the γ Z promoter suggesting a co-ordinated function between the two cis -acting elements. Two additional cis -motifs upstream of the bifactorial prolamin element have been identified: a motif with high homology to the AACA elements of rice glutelin genes and an AZM motif containing an ACGT core which binds nuclear proteins other than the Opaque 2 (O2)." @default.
- W1972238930 created "2016-06-24" @default.
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- W1972238930 creator A5074731918 @default.
- W1972238930 date "1998-10-01" @default.
- W1972238930 modified "2023-10-11" @default.
- W1972238930 title "The bifactorial endosperm box of gamma-zein gene: characterisation and function of the Pb3 and GZM cis-acting elements" @default.
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- W1972238930 doi "https://doi.org/10.1046/j.1365-313x.1998.00272.x" @default.
- W1972238930 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9807826" @default.
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