FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1972245536> ?p ?o ?g. }

• W1972245536 endingPage "96" @default.
• W1972245536 startingPage "86" @default.
• W1972245536 abstract "Maize endosperm is both a crucial contributor to world nutrition and a model system for plant developmental biology. For both aspects promoters with expression zones restricted to particular domains of the endosperm would be a valuable tool. The β-glucuronidase (Gus) reporter gene was fused to upstream fragments of three genes whose expression is limited to specific domains of the endosperm: Vacuolar pyrophosphatase 1 (Vpp1) encoding an H+ translocating vacuolar pyrophosphatase and expressed in the aleurone layer, Embryo surrounding region 6 (Esr6) encoding a defensin-like protein and expressed specifically in the embryo surrounding region (ESR) and Outer cell layer 4 (OCL4) encoding a transcription factor of the homeo domain-leucine zipper IV (HD-ZIP IV) family and expressed in the aleurone layer. The sequence analysis of the upstream fragments revealed several putative cis elements conserved either with the rice orthologues or with other maize genes sharing the same expression domain. The phenotypic characterisation of transgenic promoter-Gus lines revealed for Vpp1 a delay in the onset of GUS activity, as compared to in situ data, and a dynamic pattern shifting from the adaxial to the abaxial side of the aleurone layer. The two closely related genes Esr6a and Esr6b discovered during the cloning process diverged strongly in their upstream sequences but showed GUS signals very similar to each other and to the in situ signal: a strong, ESR-specific GUS signal at 8 days after pollination (DAP) and 16 DAP, which disappeared at 22 DAP. While Esr6b was likely the stronger promoter, a point-like signal outside the ESR on the abgerminal side at the junction of the basal endosperm transfer layer (BETL) and the aleurone layer was only found in prEsr6a-Gus plants. The GUS signal of prOCL4-Gus plants only partially reflected the in situ data; whereas OCL4 in situ signal was seen in the epidermis of male and female flowers, embryos and endosperm, prOCL4-GUS signal was only observed in female flowers and endosperm where it confirmed the high specificity for the outer cell layer and exhibited a dynamic pattern similar to Vpp1. While the upstream fragment used likely contained only some but not all the cis elements governing OCL4 expression, the fortuitously obtained truncated promoter may be of greater biotechnological interest than the complete promoter because of its more restricted expression zone that still includes the highly interesting aleurone layer." @default.
• W1972245536 created "2016-06-24" @default.
• W1972245536 creator A5003512996 @default.
• W1972245536 creator A5022608551 @default.
• W1972245536 creator A5048137300 @default.
• W1972245536 creator A5048838360 @default.
• W1972245536 creator A5050034788 @default.
• W1972245536 creator A5071588474 @default.
• W1972245536 creator A5087699183 @default.
• W1972245536 date "2010-07-01" @default.
• W1972245536 modified "2023-10-12" @default.
• W1972245536 title "The Vpp1, Esr6a, Esr6b and OCL4 promoters are active in distinct domains of maize endosperm" @default.
• W1972245536 cites W1278420624 @default.
• W1972245536 cites W1539503448 @default.
• W1972245536 cites W1552646653 @default.
• W1972245536 cites W1574030344 @default.
• W1972245536 cites W1598247816 @default.
• W1972245536 cites W1840679026 @default.
• W1972245536 cites W1983831516 @default.
• W1972245536 cites W1983868686 @default.
• W1972245536 cites W1994316583 @default.
• W1972245536 cites W1994398462 @default.
• W1972245536 cites W1998703181 @default.
• W1972245536 cites W2005595482 @default.
• W1972245536 cites W2012301467 @default.
• W1972245536 cites W2014704298 @default.
• W1972245536 cites W2044297613 @default.
• W1972245536 cites W2047063342 @default.
• W1972245536 cites W2054179899 @default.
• W1972245536 cites W2059341413 @default.
• W1972245536 cites W2079564769 @default.
• W1972245536 cites W2081916292 @default.
• W1972245536 cites W2088619532 @default.
• W1972245536 cites W2088803205 @default.
• W1972245536 cites W2088883992 @default.
• W1972245536 cites W2094360750 @default.
• W1972245536 cites W2098773881 @default.
• W1972245536 cites W2099752976 @default.
• W1972245536 cites W2099888478 @default.
• W1972245536 cites W2102660938 @default.
• W1972245536 cites W2103330614 @default.
• W1972245536 cites W2107455091 @default.
• W1972245536 cites W2110336488 @default.
• W1972245536 cites W2110641808 @default.
• W1972245536 cites W2113524952 @default.
• W1972245536 cites W2113668742 @default.
• W1972245536 cites W2125669327 @default.
• W1972245536 cites W2144617607 @default.
• W1972245536 cites W2145660214 @default.
• W1972245536 cites W2146105226 @default.
• W1972245536 cites W2150384780 @default.
• W1972245536 cites W2156640201 @default.
• W1972245536 cites W2157009395 @default.
• W1972245536 cites W2159282064 @default.
• W1972245536 cites W2163930938 @default.
• W1972245536 cites W2165098477 @default.
• W1972245536 cites W2166937127 @default.
• W1972245536 cites W2171326228 @default.
• W1972245536 doi "https://doi.org/10.1016/j.plantsci.2010.04.006" @default.
• W1972245536 hasPublicationYear "2010" @default.
• W1972245536 type Work @default.
• W1972245536 sameAs 1972245536 @default.
• W1972245536 citedByCount "4" @default.
• W1972245536 countsByYear W19722455362014 @default.
• W1972245536 countsByYear W19722455362015 @default.
• W1972245536 countsByYear W19722455362020 @default.
• W1972245536 countsByYear W19722455362021 @default.
• W1972245536 crossrefType "journal-article" @default.
• W1972245536 hasAuthorship W1972245536A5003512996 @default.
• W1972245536 hasAuthorship W1972245536A5022608551 @default.
• W1972245536 hasAuthorship W1972245536A5048137300 @default.
• W1972245536 hasAuthorship W1972245536A5048838360 @default.
• W1972245536 hasAuthorship W1972245536A5050034788 @default.
• W1972245536 hasAuthorship W1972245536A5071588474 @default.
• W1972245536 hasAuthorship W1972245536A5087699183 @default.
• W1972245536 hasConcept C101762097 @default.
• W1972245536 hasConcept C104317684 @default.
• W1972245536 hasConcept C113442657 @default.
• W1972245536 hasConcept C150194340 @default.
• W1972245536 hasConcept C161733203 @default.
• W1972245536 hasConcept C2776880369 @default.
• W1972245536 hasConcept C2780895877 @default.
• W1972245536 hasConcept C54355233 @default.
• W1972245536 hasConcept C86803240 @default.
• W1972245536 hasConcept C95444343 @default.
• W1972245536 hasConceptScore W1972245536C101762097 @default.
• W1972245536 hasConceptScore W1972245536C104317684 @default.
• W1972245536 hasConceptScore W1972245536C113442657 @default.
• W1972245536 hasConceptScore W1972245536C150194340 @default.
• W1972245536 hasConceptScore W1972245536C161733203 @default.
• W1972245536 hasConceptScore W1972245536C2776880369 @default.
• W1972245536 hasConceptScore W1972245536C2780895877 @default.
• W1972245536 hasConceptScore W1972245536C54355233 @default.
• W1972245536 hasConceptScore W1972245536C86803240 @default.
• W1972245536 hasConceptScore W1972245536C95444343 @default.
• W1972245536 hasIssue "1-2" @default.
• W1972245536 hasLocation W19722455361 @default.
• W1972245536 hasLocation W19722455362 @default.

• next