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- W1972254452 abstract "We produced antiserum to insulin-like growth factor I (IGF-I), and developed a specific and sensitive radioimmunoassay (RIA) for IGF-I using the biosynthetic IGF-I. This antiserum to IGF-I was specific for IGF-I; no crossreactivities with multiplication stimulating activity, porcine insulin or human growth hormone (hGH) were detected. The sensitivity was 10-25 pg/tube with 50% displacement at 125 pg/tube. The intra- and inter-assay coefficients of variation for IGF-I were 5.4 and 9.7%, respectively. The plasma IGF-I levels as determined by RIA in normal adults (N=46), patients with active acromegaly (N=31), and pituitary dwarfs (N=31) were 21.6±1.0, 157.3±17.0, and 2.5±0.3 ng/ml (Mean±SEM), respectively, indicating the levels were GHdependent. The plasma IGF-I levels were significantly increased from 2.2±0.2 to 26.5±3.2 ng/ml after hGH administrations for three consecutive days in five pituitary dwarfs. The IGF-I levels were low in patients with hypothyroidism and liver cirrhosis, but were normal in patients with chronic renal failure.These data confirm previous reports and this radioimmunoassay proves useful in evaluating plasma IGF-I levels." @default.
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- W1972254452 date "1986-01-01" @default.
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- W1972254452 title "Radioimmunoassay for insulin-like growth factor I(IGF-I) using biosynthetic IGF-I." @default.
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- W1972254452 doi "https://doi.org/10.1507/endocrj1954.33.795" @default.
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