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- W1972371313 abstract "The initial kinetics of the enzymatic degradation of fully deproteinized native DNA by either beef pancreas neutral deoxyribonuclease (deoxyribonucleate oligonucleotidohydrolase, EC 3.1.4.5) or hog spleen acid deoxyribonuclease (deoxyribonucleate 3′-nucleotidohydrolase, EC 3.1.4.6) were thoroughly investigated, using analytical ultracentrifugation to determine the ratio of single-strand breaks to double-strand breaks. Beef pancreas deoxyribonuclease degraded DNA in a non-random manner and behaved as a region-specific enzyme. For hog spleen deoxyribonuclease, the independence of haplotomic (formation of single strand breaks) and diplotomic (formation of double-strand breaks) mechanisms was confirmed, though predominance of the former could be demonstrated." @default.
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- W1972371313 date "1972-05-01" @default.
- W1972371313 modified "2023-09-27" @default.
- W1972371313 title "A new approach to the elucidation of the initial kinetics of neutral and acid deoxyribonucleases" @default.
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- W1972371313 doi "https://doi.org/10.1016/0005-2744(72)90342-7" @default.
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