FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1972373436> ?p ?o ?g. }

• W1972373436 endingPage "4671" @default.
• W1972373436 startingPage "4665" @default.
• W1972373436 abstract "Polyphosphate, a linear polymer of orthophosphate, is abundant in the environment and a key component in wastewater treatment and many bioremediation processes. Despite the broad relevance of polyphosphate, current methods to quantify it possess significant disadvantages. Here, we describe a new approach for the direct quantification of inorganic polyphosphate in complex natural samples. The protocol relies on the interaction between the fluorochrome 4',6-diamidino-2-phenylindole (DAPI) and dissolved polyphosphate. With the DAPI-based approach we describe, polyphosphate can be quantified at concentrations ranging from 0.5-3 microM P in a neutral-buffered freshwater matrix with an accuracy of +/-0.03 microM P. The patterns of polyphosphate concentration versus fluorescence yielded by standards exhibit no chain length dependence across polyphosphates ranging from 15-130 phosphorus units in size. Shorter length polyphosphate molecules (e.g., polyphosphate of three and five phosphorus units in length) contribute little to no signal in this approach, as these molecules react only slightly or not at all with DAPI in the concentration range tested. The presence of salt suppresses fluorescence from intermediate polyphosphate chain lengths (e.g., 15 phosphorus units) at polyphosphate concentrations ranging from 0.5-3 microM P. For longer chain lengths (e.g., 45-130 phosphorus units), this salt interference is not evident at conductivities up to approximately 10mS/cm. Our results indicate that standard polyphosphates should be stored frozen for no longer than 10-15 days to avoid inconsistent results associated with standard degradation. We have applied the fluorometric protocol to the analysis of five well-characterized natural samples to demonstrate the use of the method." @default.
• W1972373436 created "2016-06-24" @default.
• W1972373436 creator A5000455929 @default.
• W1972373436 creator A5046291649 @default.
• W1972373436 date "2010-05-27" @default.
• W1972373436 modified "2023-10-17" @default.
• W1972373436 title "Fluorometric Quantification of Natural Inorganic Polyphosphate" @default.
• W1972373436 cites W1872374211 @default.
• W1972373436 cites W1966175891 @default.
• W1972373436 cites W1976529943 @default.
• W1972373436 cites W1990167105 @default.
• W1972373436 cites W2000593151 @default.
• W1972373436 cites W2002246851 @default.
• W1972373436 cites W2012314953 @default.
• W1972373436 cites W2016816054 @default.
• W1972373436 cites W2017834179 @default.
• W1972373436 cites W2020748522 @default.
• W1972373436 cites W2023161358 @default.
• W1972373436 cites W2039903424 @default.
• W1972373436 cites W2041634433 @default.
• W1972373436 cites W2059950143 @default.
• W1972373436 cites W2062244827 @default.
• W1972373436 cites W2064018180 @default.
• W1972373436 cites W2068597492 @default.
• W1972373436 cites W2068903500 @default.
• W1972373436 cites W2078012795 @default.
• W1972373436 cites W2096011303 @default.
• W1972373436 cites W2101734599 @default.
• W1972373436 cites W2107572522 @default.
• W1972373436 cites W2114984742 @default.
• W1972373436 cites W2118716661 @default.
• W1972373436 cites W2120012985 @default.
• W1972373436 cites W2125837377 @default.
• W1972373436 cites W2135129206 @default.
• W1972373436 cites W2139054237 @default.
• W1972373436 cites W2139347632 @default.
• W1972373436 cites W2141450887 @default.
• W1972373436 cites W2158200489 @default.
• W1972373436 cites W2159583014 @default.
• W1972373436 cites W2161220379 @default.
• W1972373436 cites W2164119089 @default.
• W1972373436 cites W2165387875 @default.
• W1972373436 cites W2312935062 @default.
• W1972373436 cites W4237887470 @default.
• W1972373436 cites W2133089084 @default.
• W1972373436 doi "https://doi.org/10.1021/es100191h" @default.
• W1972373436 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20507063" @default.
• W1972373436 hasPublicationYear "2010" @default.
• W1972373436 type Work @default.
• W1972373436 sameAs 1972373436 @default.
• W1972373436 citedByCount "48" @default.
• W1972373436 countsByYear W19723734362012 @default.
• W1972373436 countsByYear W19723734362013 @default.
• W1972373436 countsByYear W19723734362014 @default.
• W1972373436 countsByYear W19723734362015 @default.
• W1972373436 countsByYear W19723734362016 @default.
• W1972373436 countsByYear W19723734362017 @default.
• W1972373436 countsByYear W19723734362018 @default.
• W1972373436 countsByYear W19723734362019 @default.
• W1972373436 countsByYear W19723734362020 @default.
• W1972373436 countsByYear W19723734362021 @default.
• W1972373436 countsByYear W19723734362022 @default.
• W1972373436 countsByYear W19723734362023 @default.
• W1972373436 crossrefType "journal-article" @default.
• W1972373436 hasAuthorship W1972373436A5000455929 @default.
• W1972373436 hasAuthorship W1972373436A5046291649 @default.
• W1972373436 hasConcept C121332964 @default.
• W1972373436 hasConcept C178790620 @default.
• W1972373436 hasConcept C185592680 @default.
• W1972373436 hasConcept C2777132085 @default.
• W1972373436 hasConcept C2778038992 @default.
• W1972373436 hasConcept C43617362 @default.
• W1972373436 hasConcept C510538283 @default.
• W1972373436 hasConcept C55493867 @default.
• W1972373436 hasConcept C62520636 @default.
• W1972373436 hasConcept C91881484 @default.
• W1972373436 hasConceptScore W1972373436C121332964 @default.
• W1972373436 hasConceptScore W1972373436C178790620 @default.
• W1972373436 hasConceptScore W1972373436C185592680 @default.
• W1972373436 hasConceptScore W1972373436C2777132085 @default.
• W1972373436 hasConceptScore W1972373436C2778038992 @default.
• W1972373436 hasConceptScore W1972373436C43617362 @default.
• W1972373436 hasConceptScore W1972373436C510538283 @default.
• W1972373436 hasConceptScore W1972373436C55493867 @default.
• W1972373436 hasConceptScore W1972373436C62520636 @default.
• W1972373436 hasConceptScore W1972373436C91881484 @default.
• W1972373436 hasIssue "12" @default.
• W1972373436 hasLocation W19723734361 @default.
• W1972373436 hasLocation W19723734362 @default.
• W1972373436 hasOpenAccess W1972373436 @default.
• W1972373436 hasPrimaryLocation W19723734361 @default.
• W1972373436 hasRelatedWork W1515384936 @default.
• W1972373436 hasRelatedWork W2016889584 @default.
• W1972373436 hasRelatedWork W2033537114 @default.
• W1972373436 hasRelatedWork W2042899294 @default.
• W1972373436 hasRelatedWork W2046397866 @default.
• W1972373436 hasRelatedWork W2051764094 @default.
• W1972373436 hasRelatedWork W2060278151 @default.

• next