FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1972373490> ?p ?o ?g. }

• W1972373490 endingPage "1927" @default.
• W1972373490 startingPage "1917" @default.
• W1972373490 abstract "Adenine deaminase (ADE) catalyzes the conversion of adenine to hypoxanthine and ammonia. The enzyme isolated from Escherichia coli using standard expression conditions was low for the deamination of adenine (kcat = 2.0 s−1; kcat/Km = 2.5 × 103 M−1 s−1). However, when iron was sequestered with a metal chelator and the growth medium was supplemented with Mn2+ prior to induction, the purified enzyme was substantially more active for the deamination of adenine with kcat and kcat/Km values of 200 s−1 and 5 × 105 M−1 s−1, respectively. The apoenzyme was prepared and reconstituted with Fe2+, Zn2+, or Mn2+. In each case, two enzyme equivalents of metal were necessary for reconstitution of the deaminase activity. This work provides the first example of any member of the deaminase subfamily of the amidohydrolase superfamily to utilize a binuclear metal center for the catalysis of a deamination reaction. [FeII/FeII]-ADE was oxidized to [FeIII/FeIII]-ADE with ferricyanide with inactivation of the deaminase activity. Reducing [FeIII/FeIII]-ADE with dithionite restored the deaminase activity, and thus, the diferrous form of the enzyme is essential for catalytic activity. No evidence of spin coupling between metal ions was evident by electron paramagnetic resonance or Mössbauer spectroscopy. The three-dimensional structure of adenine deaminase from Agrobacterium tumefaciens (Atu4426) was determined by X-ray crystallography at 2.2 Å resolution, and adenine was modeled into the active site on the basis of homology to other members of the amidohydrolase superfamily. On the basis of the model of the adenine−ADE complex and subsequent mutagenesis experiments, the roles for each of the highly conserved residues were proposed. Solvent isotope effects, pH−rate profiles, and solvent viscosity were utilized to propose a chemical reaction mechanism and the identity of the rate-limiting steps." @default.
• W1972373490 created "2016-06-24" @default.
• W1972373490 creator A5002108565 @default.
• W1972373490 creator A5002277619 @default.
• W1972373490 creator A5014074389 @default.
• W1972373490 creator A5020068139 @default.
• W1972373490 creator A5028845140 @default.
• W1972373490 creator A5052850826 @default.
• W1972373490 creator A5068609159 @default.
• W1972373490 creator A5068648875 @default.
• W1972373490 creator A5069253317 @default.
• W1972373490 creator A5075327940 @default.
• W1972373490 creator A5078000036 @default.
• W1972373490 date "2011-02-04" @default.
• W1972373490 modified "2023-10-18" @default.
• W1972373490 title "Catalytic Mechanism and Three-Dimensional Structure of Adenine Deaminase^," @default.
• W1972373490 cites W1539796472 @default.
• W1972373490 cites W1591680814 @default.
• W1972373490 cites W1969672293 @default.
• W1972373490 cites W1972951063 @default.
• W1972373490 cites W1975691556 @default.
• W1972373490 cites W1985203726 @default.
• W1972373490 cites W1986618078 @default.
• W1972373490 cites W1989773022 @default.
• W1972373490 cites W1995895217 @default.
• W1972373490 cites W2003687681 @default.
• W1972373490 cites W2004962085 @default.
• W1972373490 cites W2015304573 @default.
• W1972373490 cites W2035940253 @default.
• W1972373490 cites W2039478231 @default.
• W1972373490 cites W2039862991 @default.
• W1972373490 cites W2048350041 @default.
• W1972373490 cites W2048425759 @default.
• W1972373490 cites W2051350436 @default.
• W1972373490 cites W2055161377 @default.
• W1972373490 cites W2065283382 @default.
• W1972373490 cites W2074004794 @default.
• W1972373490 cites W2074043893 @default.
• W1972373490 cites W2077451485 @default.
• W1972373490 cites W2086795417 @default.
• W1972373490 cites W2093264253 @default.
• W1972373490 cites W2093436341 @default.
• W1972373490 cites W2099900074 @default.
• W1972373490 cites W2104017463 @default.
• W1972373490 cites W2112161333 @default.
• W1972373490 cites W2136758633 @default.
• W1972373490 cites W2140178909 @default.
• W1972373490 cites W2160216877 @default.
• W1972373490 cites W2162220273 @default.
• W1972373490 cites W2171234651 @default.
• W1972373490 cites W4294216483 @default.
• W1972373490 cites W4302036178 @default.
• W1972373490 doi "https://doi.org/10.1021/bi101788n" @default.
• W1972373490 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3059353" @default.
• W1972373490 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21247091" @default.
• W1972373490 hasPublicationYear "2011" @default.
• W1972373490 type Work @default.
• W1972373490 sameAs 1972373490 @default.
• W1972373490 citedByCount "41" @default.
• W1972373490 countsByYear W19723734902012 @default.
• W1972373490 countsByYear W19723734902013 @default.
• W1972373490 countsByYear W19723734902014 @default.
• W1972373490 countsByYear W19723734902015 @default.
• W1972373490 countsByYear W19723734902016 @default.
• W1972373490 countsByYear W19723734902017 @default.
• W1972373490 countsByYear W19723734902018 @default.
• W1972373490 countsByYear W19723734902019 @default.
• W1972373490 countsByYear W19723734902020 @default.
• W1972373490 countsByYear W19723734902021 @default.
• W1972373490 countsByYear W19723734902022 @default.
• W1972373490 crossrefType "journal-article" @default.
• W1972373490 hasAuthorship W1972373490A5002108565 @default.
• W1972373490 hasAuthorship W1972373490A5002277619 @default.
• W1972373490 hasAuthorship W1972373490A5014074389 @default.
• W1972373490 hasAuthorship W1972373490A5020068139 @default.
• W1972373490 hasAuthorship W1972373490A5028845140 @default.
• W1972373490 hasAuthorship W1972373490A5052850826 @default.
• W1972373490 hasAuthorship W1972373490A5068609159 @default.
• W1972373490 hasAuthorship W1972373490A5068648875 @default.
• W1972373490 hasAuthorship W1972373490A5069253317 @default.
• W1972373490 hasAuthorship W1972373490A5075327940 @default.
• W1972373490 hasAuthorship W1972373490A5078000036 @default.
• W1972373490 hasBestOaLocation W19723734902 @default.
• W1972373490 hasConcept C145782189 @default.
• W1972373490 hasConcept C181199279 @default.
• W1972373490 hasConcept C185592680 @default.
• W1972373490 hasConcept C2779485144 @default.
• W1972373490 hasConcept C41183919 @default.
• W1972373490 hasConcept C55493867 @default.
• W1972373490 hasConcept C56856141 @default.
• W1972373490 hasConcept C71240020 @default.
• W1972373490 hasConceptScore W1972373490C145782189 @default.
• W1972373490 hasConceptScore W1972373490C181199279 @default.
• W1972373490 hasConceptScore W1972373490C185592680 @default.
• W1972373490 hasConceptScore W1972373490C2779485144 @default.
• W1972373490 hasConceptScore W1972373490C41183919 @default.
• W1972373490 hasConceptScore W1972373490C55493867 @default.
• W1972373490 hasConceptScore W1972373490C56856141 @default.

• next