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• W1972407740 abstract "1.1. Studies were made on sodium and potassium regulation in Blepharisma intermedium. Ionic levels were expressed in terms of μM/g protein. Sodium and potassium were measured with a Beckman DU spectrophotometer adapted for use as a flame photometer. Protein was measured by the Lowry method.2.2. The ionic levels of Blepharisma in different concentrations of a modified Taylor-Strickland balanced salt solution were studied. High internal potassium levels existed in all concentrations studied. Blepharisma maintained low internal sodium levels up to 20 × balanced salt solution. Above this concentration, internal sodium levels increased with external levels although the internal level was always maintained about 37 mM/1 below the environment.3.3. Blepharisma placed at 5 °C gain sodium and lose potassium in about equal amounts. Sodium is extruded and potassium accumulated when the animals are brought back to room temperature. Recovery in a K-free solution slows the rate of sodium extrusion.4.4. Inhibitors of oxidative metabolism (sodium azide, dinitrophenol, and sodium cyanide) and of glycolysis (sodium fluoride) produce an increase in internal sodium levels. Usually these inhibitors have little effect on the internal potassium levels but occasionally produce a slight, unexpected increase in internal potassium.5.5. Ouabain, an inhibitor of the sodium pump, produced a large increase in internal sodium and a small increase in internal potassium.6.6. Gum tragacanth and tetrodotoxin have little effect on sodium and potassium levels.7.7. Blepharisma was immobilized in salt solutions containing high sodium concentrations but not in solutions containing high potassium chloride, magnesium chloride, magnesium sulfate, calcium chloride, or sucrose. Sodium azide and dinitrophenol immobilize Blepharisma at low inhibitor concentrations. Other metabolic inhibitors immobilize Blepharisma at concentrations at which internal sodium has increased." @default.
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• W1972407740 date "1970-08-01" @default.
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• W1972407740 title "Sodium and potassium levels in Blepharisma intermedium" @default.
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• W1972407740 doi "https://doi.org/10.1016/0014-4827(70)90444-1" @default.
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