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- W1972500392 abstract "Kinetoplast DNA (ϱ = 1.703 g/ml.) was isolated by preparative cesium chloride ultracentrifugation in a fixed-angle rotor from total cell DNA of Leishmania tarentolae and examined in terms of sedimentation properties, melting characteristics, and appearance in the electron microscope. It consisted of several molecular types, either free or bound together in associations of variable size: minicircles (molecular weight = 0.56 ± 0.03 × 106), catenated minicircles, “figure 8” molecules, and long molecules. The associations seem to be held together by the long molecules threading through the smaller circles and catenanes. The large associations could be broken down by sonication, DNase II-treatment, or shear forces. Minicircles, catenated dimers, trimers, and small linear fragments were separated on preparative sucrose gradients of sonicated DNA, and S20,w values were assigned to each molecular type by band sedimentation in the analytical ultracentrifuge. The kinetoplast DNA was found to be conserved during at least 4.8 cell divisions. There are more than 14 DNA units which segregate independently of each other on cell division. Nuclear DNA (ϱ = 1.716 g/ml.) consisted of long, linear molecules, and had a monophasic melting curve. Kinetoplast DNA did not denature irreversibly until the closed circular molecules were nicked or broken. A preliminary model was presented for the molecular architecture of the kinetoplast DNA structure in situ from the fact that the width of the kinetoplast DNA found in thin sections is equal to the diameter of a minicircle." @default.
- W1972500392 created "2016-06-24" @default.
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- W1972500392 date "1971-03-01" @default.
- W1972500392 modified "2023-09-26" @default.
- W1972500392 title "Isolation and characterization of kinetoplast DNA from Leishmania tarentolae" @default.
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- W1972500392 doi "https://doi.org/10.1016/0022-2836(71)90394-9" @default.
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