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- W1972503665 abstract "Abstract Peripheral blood lymphocytes from normal individuals and patients with ALL, AML, CLL, CML and Hodgkin's disease were compared in the fresh and frozen-thawed states. The cells were frozen in DMSO by either a controlled (1 °C/min) or uncontrolled rate freezing process and stored in liquid nitrogen (−196 °C) for 1 day to 12.5 years. No significant difference was found between fresh and frozen-thawed cells of normal or leukemic subjects in the observed percentage of spontaneous E-rosette formation. Leukemic cells frozen by the controlled rate method and preserved in liquid nitrogen for 12.5 years were found to be fully capable of rosette formation, and when stained with Trypan Blue, the majority (> 70%) were found to be viable." @default.
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- W1972503665 date "1978-02-01" @default.
- W1972503665 modified "2023-10-16" @default.
- W1972503665 title "E-rosette formation of fresh versus frozen-thawed lymphocytes from leukemic patients and normal individuals" @default.
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- W1972503665 doi "https://doi.org/10.1016/0011-2240(78)90008-1" @default.
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