Matches in SemOpenAlex for { <https://semopenalex.org/work/W1972504486> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W1972504486 endingPage "1862" @default.
- W1972504486 startingPage "1859" @default.
- W1972504486 abstract "The effects of some divalent cations on protoplast transfection mediated by polyethylene glycol of Lactobacillus casei ATCC 27092 by PL-1 phage DNA in 50 mM Tris-maleate buffer (pH 6.0) were investigated. The efficiency of transfection increased about 30 times in the presence of 10 mM Ca2+. Sr2+ increased the transfection rate as well, but Ba2+, Mn2+, and Mg2+ did not. Co2+ and Zn2+ inhibited transfection. The simultaneous use of Ca2+ and Mg2+ increased the transfection efficiency. Impairment of transfection caused by lack of Ca2+ could not be reversed by the addition of Ca2+ later. A decrease in the Ca2+ concentration to an ineffective level before transfection ended immediately inhibited transfection. Protoplasts were transfected with a phage adsorption mutant resistant to PL-1, also, and these metal ions had the same effect. Multiplication of phages in the transfected protoplasts was independent of the presence or absence of calcium ions. Calcium ions seemed to be involved in the entry of PL-1 DNA into the host protoplasts." @default.
- W1972504486 created "2016-06-24" @default.
- W1972504486 creator A5004743260 @default.
- W1972504486 creator A5036777616 @default.
- W1972504486 creator A5070575959 @default.
- W1972504486 creator A5085501730 @default.
- W1972504486 date "1992-01-01" @default.
- W1972504486 modified "2023-10-17" @default.
- W1972504486 title "Calcium Requirement for Protoplast Transfection Mediated by Polyethylene Glycol ofLactobacillus caseiby PL-1 Phage DNA" @default.
- W1972504486 doi "https://doi.org/10.1271/bbb.56.1859" @default.
- W1972504486 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1369080" @default.
- W1972504486 hasPublicationYear "1992" @default.
- W1972504486 type Work @default.
- W1972504486 sameAs 1972504486 @default.
- W1972504486 citedByCount "8" @default.
- W1972504486 countsByYear W19725044862014 @default.
- W1972504486 crossrefType "journal-article" @default.
- W1972504486 hasAuthorship W1972504486A5004743260 @default.
- W1972504486 hasAuthorship W1972504486A5036777616 @default.
- W1972504486 hasAuthorship W1972504486A5070575959 @default.
- W1972504486 hasAuthorship W1972504486A5085501730 @default.
- W1972504486 hasConcept C100544194 @default.
- W1972504486 hasConcept C104317684 @default.
- W1972504486 hasConcept C12554922 @default.
- W1972504486 hasConcept C143065580 @default.
- W1972504486 hasConcept C153911025 @default.
- W1972504486 hasConcept C173749725 @default.
- W1972504486 hasConcept C178790620 @default.
- W1972504486 hasConcept C185592680 @default.
- W1972504486 hasConcept C2776662696 @default.
- W1972504486 hasConcept C2776964284 @default.
- W1972504486 hasConcept C2777658833 @default.
- W1972504486 hasConcept C519063684 @default.
- W1972504486 hasConcept C54009773 @default.
- W1972504486 hasConcept C552990157 @default.
- W1972504486 hasConcept C55493867 @default.
- W1972504486 hasConcept C74884574 @default.
- W1972504486 hasConcept C86803240 @default.
- W1972504486 hasConceptScore W1972504486C100544194 @default.
- W1972504486 hasConceptScore W1972504486C104317684 @default.
- W1972504486 hasConceptScore W1972504486C12554922 @default.
- W1972504486 hasConceptScore W1972504486C143065580 @default.
- W1972504486 hasConceptScore W1972504486C153911025 @default.
- W1972504486 hasConceptScore W1972504486C173749725 @default.
- W1972504486 hasConceptScore W1972504486C178790620 @default.
- W1972504486 hasConceptScore W1972504486C185592680 @default.
- W1972504486 hasConceptScore W1972504486C2776662696 @default.
- W1972504486 hasConceptScore W1972504486C2776964284 @default.
- W1972504486 hasConceptScore W1972504486C2777658833 @default.
- W1972504486 hasConceptScore W1972504486C519063684 @default.
- W1972504486 hasConceptScore W1972504486C54009773 @default.
- W1972504486 hasConceptScore W1972504486C552990157 @default.
- W1972504486 hasConceptScore W1972504486C55493867 @default.
- W1972504486 hasConceptScore W1972504486C74884574 @default.
- W1972504486 hasConceptScore W1972504486C86803240 @default.
- W1972504486 hasIssue "11" @default.
- W1972504486 hasLocation W19725044861 @default.
- W1972504486 hasLocation W19725044862 @default.
- W1972504486 hasOpenAccess W1972504486 @default.
- W1972504486 hasPrimaryLocation W19725044861 @default.
- W1972504486 hasRelatedWork W10416008 @default.
- W1972504486 hasRelatedWork W1972504486 @default.
- W1972504486 hasRelatedWork W1996088859 @default.
- W1972504486 hasRelatedWork W2018586319 @default.
- W1972504486 hasRelatedWork W2066255734 @default.
- W1972504486 hasRelatedWork W2070825180 @default.
- W1972504486 hasRelatedWork W2071887022 @default.
- W1972504486 hasRelatedWork W2319816120 @default.
- W1972504486 hasRelatedWork W2464716460 @default.
- W1972504486 hasRelatedWork W1796545317 @default.
- W1972504486 hasVolume "56" @default.
- W1972504486 isParatext "false" @default.
- W1972504486 isRetracted "false" @default.
- W1972504486 magId "1972504486" @default.
- W1972504486 workType "article" @default.