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• W1972505722 abstract "Abstract Co-injection of zygotes with Cas9 mRNA and sgRNA has been proven to be an efficient gene-editing strategy for genome modification of different species. Genetic engineering in pigs holds a great promise in biomedical research. By co-injection of one-cell stage embryos with Cas9 mRNA and Npc1l1 sgRNA, we achieved precise Npc1l1 targeting in Chinese Bama miniature pigs at the efficiency as high as 100%. Meanwhile, we carefully analyzed the Npc1l1 sgRNA:Cas9-mediated on- and off-target mutations in various somatic tissues and ovaries and demonstrated that injection of zygotes with Cas9 mRNA and sgRNA is an efficient and reliable approach for generation of gene-modified pigs." @default.
• W1972505722 created "2016-06-24" @default.
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• W1972505722 date "2015-02-05" @default.
• W1972505722 modified "2023-10-14" @default.
• W1972505722 title "Efficient generation of gene-modified pigs via injection of zygote with Cas9/sgRNA" @default.
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• W1972505722 doi "https://doi.org/10.1038/srep08256" @default.
• W1972505722 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4317696" @default.
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