Matches in SemOpenAlex for { <https://semopenalex.org/work/W1972509634> ?p ?o ?g. }
- W1972509634 endingPage "16417" @default.
- W1972509634 startingPage "16410" @default.
- W1972509634 abstract "Blue fluorescent proteins (BFPs) offer visualization of protein location and behavior, but often suffer from high autofluorescent background and poor signal discrimination. Through dual-laser excitation of bright and photoinduced dark states, mutations to the residues surrounding the BFP chromophore enable long-wavelength optical modulation of BFP emission. Such dark state engineering enables violet-excited blue emission to be increased upon lower energy, green coillumination. Turning this green coillumination on and off at a specific frequency dynamically modulates collected blue fluorescence without generating additional background. Interpreted as transient photoconversion between neutral cis and anionic trans chromophoric forms, mutations tune photoisomerization and ground state tautomerizations to enable long-wavelength depopulation of the millisecond-lived, spectrally shifted dark states. Single mutations to the tyrosine-based blue fluorescent protein T203V/S205V exhibit enhanced modulation depth and varied frequency. Importantly, analogous single point mutations in the nonmodulatable BFP, mKalama1, creates a modulatable variant. Building modulatable BFPs offers opportunities for improved BFP signal discrimination vs background, greatly enhancing their utility." @default.
- W1972509634 created "2016-06-24" @default.
- W1972509634 creator A5027096922 @default.
- W1972509634 creator A5035802631 @default.
- W1972509634 creator A5035865050 @default.
- W1972509634 creator A5062390256 @default.
- W1972509634 creator A5067927228 @default.
- W1972509634 creator A5075784142 @default.
- W1972509634 creator A5080348602 @default.
- W1972509634 creator A5086974830 @default.
- W1972509634 creator A5090098931 @default.
- W1972509634 date "2013-10-25" @default.
- W1972509634 modified "2023-09-24" @default.
- W1972509634 title "Optically Modulatable Blue Fluorescent Proteins" @default.
- W1972509634 cites W1657499324 @default.
- W1972509634 cites W1958433286 @default.
- W1972509634 cites W1968940874 @default.
- W1972509634 cites W1969566306 @default.
- W1972509634 cites W1974642741 @default.
- W1972509634 cites W1977902910 @default.
- W1972509634 cites W1984187556 @default.
- W1972509634 cites W1986593253 @default.
- W1972509634 cites W1992858116 @default.
- W1972509634 cites W1993647922 @default.
- W1972509634 cites W1997279747 @default.
- W1972509634 cites W1997835527 @default.
- W1972509634 cites W2000837237 @default.
- W1972509634 cites W2011218016 @default.
- W1972509634 cites W2014732296 @default.
- W1972509634 cites W2024526152 @default.
- W1972509634 cites W2029409130 @default.
- W1972509634 cites W2030239671 @default.
- W1972509634 cites W2030693318 @default.
- W1972509634 cites W2030695915 @default.
- W1972509634 cites W2035031049 @default.
- W1972509634 cites W2036742051 @default.
- W1972509634 cites W2038756774 @default.
- W1972509634 cites W2049989350 @default.
- W1972509634 cites W2054455698 @default.
- W1972509634 cites W2066324902 @default.
- W1972509634 cites W2069496952 @default.
- W1972509634 cites W2073442506 @default.
- W1972509634 cites W2080005381 @default.
- W1972509634 cites W2089525686 @default.
- W1972509634 cites W2090212447 @default.
- W1972509634 cites W2098103683 @default.
- W1972509634 cites W2104109138 @default.
- W1972509634 cites W2108407770 @default.
- W1972509634 cites W2109972707 @default.
- W1972509634 cites W2116435209 @default.
- W1972509634 cites W2124341702 @default.
- W1972509634 cites W2127068865 @default.
- W1972509634 cites W2127632299 @default.
- W1972509634 cites W2137853644 @default.
- W1972509634 cites W2139414608 @default.
- W1972509634 cites W2143094150 @default.
- W1972509634 cites W2151228364 @default.
- W1972509634 cites W2159916641 @default.
- W1972509634 cites W2161996750 @default.
- W1972509634 cites W2163024465 @default.
- W1972509634 cites W2167353442 @default.
- W1972509634 doi "https://doi.org/10.1021/ja405459b" @default.
- W1972509634 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3885243" @default.
- W1972509634 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24099419" @default.
- W1972509634 hasPublicationYear "2013" @default.
- W1972509634 type Work @default.
- W1972509634 sameAs 1972509634 @default.
- W1972509634 citedByCount "33" @default.
- W1972509634 countsByYear W19725096342014 @default.
- W1972509634 countsByYear W19725096342015 @default.
- W1972509634 countsByYear W19725096342016 @default.
- W1972509634 countsByYear W19725096342017 @default.
- W1972509634 countsByYear W19725096342018 @default.
- W1972509634 countsByYear W19725096342019 @default.
- W1972509634 countsByYear W19725096342020 @default.
- W1972509634 countsByYear W19725096342021 @default.
- W1972509634 countsByYear W19725096342023 @default.
- W1972509634 crossrefType "journal-article" @default.
- W1972509634 hasAuthorship W1972509634A5027096922 @default.
- W1972509634 hasAuthorship W1972509634A5035802631 @default.
- W1972509634 hasAuthorship W1972509634A5035865050 @default.
- W1972509634 hasAuthorship W1972509634A5062390256 @default.
- W1972509634 hasAuthorship W1972509634A5067927228 @default.
- W1972509634 hasAuthorship W1972509634A5075784142 @default.
- W1972509634 hasAuthorship W1972509634A5080348602 @default.
- W1972509634 hasAuthorship W1972509634A5086974830 @default.
- W1972509634 hasAuthorship W1972509634A5090098931 @default.
- W1972509634 hasBestOaLocation W19725096342 @default.
- W1972509634 hasConcept C104317684 @default.
- W1972509634 hasConcept C120665830 @default.
- W1972509634 hasConcept C121332964 @default.
- W1972509634 hasConcept C123079801 @default.
- W1972509634 hasConcept C12554922 @default.
- W1972509634 hasConcept C126661725 @default.
- W1972509634 hasConcept C131918908 @default.
- W1972509634 hasConcept C142613039 @default.
- W1972509634 hasConcept C161790260 @default.
- W1972509634 hasConcept C181500209 @default.