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- W1972536612 abstract "In search of antimicrobial peptides in the tunicate Ciona intestinalis, we used the recently completed genome project and the substantial number of expressed sequence tag (EST) data available as a screening matrix. By this means, we identified a putative gene family that exhibits several structural features typical of antimicrobial peptides. We designed and synthesized a peptide corresponding to the core region of a member of this peptide family, which is predicted to adopt an amphipathic α-helical structure. The synthetic peptide exerted potent antimicrobial activity against a variety of bacteria and against the yeast Candida albicans but was not cytolytic for mammalian erythrocytes. Moreover, by employing a non-cell-permeable fluorescent dye it became evident that the peptide kills bacteria by permeabilizing their cytoplasmic membranes. Using the synthetic peptide as an antigen, we generated specific antibodies and localized the natural parent molecule to a compartment of a distinct hemocyte type, the univacuolar refractile granulocytes. As C. intestinalis apparently does not possess gene products that resemble well-known antimicrobial peptides of tunicates and of other animals, the aforementioned peptide family may represent a potent armamentarium of the hemocytes to combat microbial infection in sea squirts." @default.
- W1972536612 created "2016-06-24" @default.
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- W1972536612 date "2008-01-01" @default.
- W1972536612 modified "2023-09-27" @default.
- W1972536612 title "A reverse search for antimicrobial peptides in Ciona intestinalis: Identification of a gene family expressed in hemocytes and evaluation of activity" @default.
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- W1972536612 doi "https://doi.org/10.1016/j.dci.2007.06.003" @default.
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