FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1972551552> ?p ?o ?g. }

• W1972551552 endingPage "2733" @default.
• W1972551552 startingPage "2725" @default.
• W1972551552 abstract "Quantitative proteomics using isobaric labeling typically involves sample digestion, peptide-level labeling and 2D LC-MS/MS. Proteomic analysis of complex samples can potentially be performed more comprehensively with GeLC-MS/MS. However, combining this approach with peptide-level labeling of multiple in-gel digests from entirely sectioned gel lanes can introduce many points of variation and adversely affect the final quantitative accuracy. Alternatively, samples labeled with isobaric tags at the protein level can be combined and analyzed by GeLC-MS/MS as a single gel lane. A caveat to this strategy is that only lysine residues are labeled, which might limit protein digestion and quantitation of peptides. Here we have compared a protein-level labeling GeLC-MS/MS strategy with a peptide-level labeling 2D LC-MS/MS approach, using mouse hippocampus synaptosomes and isobaric tandem mass tags. Protein-level labeling enabled the identification of 3 times more proteins (697 versus 241) than did peptide-level labeling, and importantly for quantitation, twice as many proteins with labeled peptides (480 versus 232) were identified. Preliminary in silico analysis also suggested the alternative use of Asp-N to trypsin to circumvent the interference of lysine labeling on protein digestion. Use of Asp-N resulted in the effective analysis of fewer peptides than with trypsin for the protein-level approach (1677 versus 3131), but yielded a similar quantitative proteomic coverage in terms of both peptides (1150 versus 1181) and proteins (448 versus 480). Taken together, these experiments demonstrate that protein-level labeling combined with GeLC-MS/MS is an effective strategy for the multiplexed quantitation of synaptosomal preparations, and may also be applicable to samples of a similar proteomic complexity and dynamic range of protein abundance." @default.
• W1972551552 created "2016-06-24" @default.
• W1972551552 creator A5017915428 @default.
• W1972551552 creator A5045885328 @default.
• W1972551552 creator A5075340919 @default.
• W1972551552 creator A5080845108 @default.
• W1972551552 creator A5085793083 @default.
• W1972551552 date "2010-03-30" @default.
• W1972551552 modified "2023-10-16" @default.
• W1972551552 title "Comparison of a Protein-Level and Peptide-Level Labeling Strategy for Quantitative Proteomics of Synaptosomes Using Isobaric Tags" @default.
• W1972551552 cites W1544474985 @default.
• W1972551552 cites W1971147414 @default.
• W1972551552 cites W1972055109 @default.
• W1972551552 cites W1987848964 @default.
• W1972551552 cites W1997504014 @default.
• W1972551552 cites W2000685126 @default.
• W1972551552 cites W2008141792 @default.
• W1972551552 cites W2015556811 @default.
• W1972551552 cites W2024923641 @default.
• W1972551552 cites W2025422229 @default.
• W1972551552 cites W2044336832 @default.
• W1972551552 cites W2048280438 @default.
• W1972551552 cites W2063001525 @default.
• W1972551552 cites W2088253044 @default.
• W1972551552 cites W2111208057 @default.
• W1972551552 cites W2112078820 @default.
• W1972551552 cites W2114140281 @default.
• W1972551552 cites W2120016245 @default.
• W1972551552 cites W2127325743 @default.
• W1972551552 cites W2140946052 @default.
• W1972551552 cites W2150015438 @default.
• W1972551552 cites W2157592832 @default.
• W1972551552 cites W2160774768 @default.
• W1972551552 cites W2167791125 @default.
• W1972551552 doi "https://doi.org/10.1021/pr900627e" @default.
• W1972551552 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20218731" @default.
• W1972551552 hasPublicationYear "2010" @default.
• W1972551552 type Work @default.
• W1972551552 sameAs 1972551552 @default.
• W1972551552 citedByCount "27" @default.
• W1972551552 countsByYear W19725515522012 @default.
• W1972551552 countsByYear W19725515522013 @default.
• W1972551552 countsByYear W19725515522014 @default.
• W1972551552 countsByYear W19725515522017 @default.
• W1972551552 countsByYear W19725515522018 @default.
• W1972551552 countsByYear W19725515522019 @default.
• W1972551552 countsByYear W19725515522020 @default.
• W1972551552 countsByYear W19725515522021 @default.
• W1972551552 countsByYear W19725515522023 @default.
• W1972551552 crossrefType "journal-article" @default.
• W1972551552 hasAuthorship W1972551552A5017915428 @default.
• W1972551552 hasAuthorship W1972551552A5045885328 @default.
• W1972551552 hasAuthorship W1972551552A5075340919 @default.
• W1972551552 hasAuthorship W1972551552A5080845108 @default.
• W1972551552 hasAuthorship W1972551552A5085793083 @default.
• W1972551552 hasConcept C104317684 @default.
• W1972551552 hasConcept C104397665 @default.
• W1972551552 hasConcept C112979427 @default.
• W1972551552 hasConcept C162356407 @default.
• W1972551552 hasConcept C181199279 @default.
• W1972551552 hasConcept C184185385 @default.
• W1972551552 hasConcept C185592680 @default.
• W1972551552 hasConcept C202157981 @default.
• W1972551552 hasConcept C2779281246 @default.
• W1972551552 hasConcept C2780340462 @default.
• W1972551552 hasConcept C31827203 @default.
• W1972551552 hasConcept C43617362 @default.
• W1972551552 hasConcept C46111723 @default.
• W1972551552 hasConcept C55493867 @default.
• W1972551552 hasConcept C73090800 @default.
• W1972551552 hasConcept C80311884 @default.
• W1972551552 hasConcept C86153913 @default.
• W1972551552 hasConcept C86803240 @default.
• W1972551552 hasConceptScore W1972551552C104317684 @default.
• W1972551552 hasConceptScore W1972551552C104397665 @default.
• W1972551552 hasConceptScore W1972551552C112979427 @default.
• W1972551552 hasConceptScore W1972551552C162356407 @default.
• W1972551552 hasConceptScore W1972551552C181199279 @default.
• W1972551552 hasConceptScore W1972551552C184185385 @default.
• W1972551552 hasConceptScore W1972551552C185592680 @default.
• W1972551552 hasConceptScore W1972551552C202157981 @default.
• W1972551552 hasConceptScore W1972551552C2779281246 @default.
• W1972551552 hasConceptScore W1972551552C2780340462 @default.
• W1972551552 hasConceptScore W1972551552C31827203 @default.
• W1972551552 hasConceptScore W1972551552C43617362 @default.
• W1972551552 hasConceptScore W1972551552C46111723 @default.
• W1972551552 hasConceptScore W1972551552C55493867 @default.
• W1972551552 hasConceptScore W1972551552C73090800 @default.
• W1972551552 hasConceptScore W1972551552C80311884 @default.
• W1972551552 hasConceptScore W1972551552C86153913 @default.
• W1972551552 hasConceptScore W1972551552C86803240 @default.
• W1972551552 hasIssue "5" @default.
• W1972551552 hasLocation W19725515521 @default.
• W1972551552 hasLocation W19725515522 @default.
• W1972551552 hasOpenAccess W1972551552 @default.
• W1972551552 hasPrimaryLocation W19725515521 @default.
• W1972551552 hasRelatedWork W198236498 @default.
• W1972551552 hasRelatedWork W1993363847 @default.

• next