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• W1972602940 abstract "Ataxia telangiectasia (AT) is an autosomal recessive disorder characterized by numerous clinical and cellular features. The pleiotropic nature of the AT syndrome attests to the multiple roles of ATM, the protein codified by the gene altered in AT patients. We investigated if different mutations of ATM could reflect on different alterations of nuclear architecture and chromatin organization. We selected three lymphoblastoid cell lines isolated from AT patients affected by different mutations of ATM gene and one healthy control. We characterized the in situ chromatin structure of each cell line by a biophysical approach: (1) we evaluated the rearrangements of the chromatin domains at the level of single cell by quantitative fluorescence microscopy; (2) we analysed the changes of the average chromatin condensation by differential scanning calorimetry. The results show that the three different ATM mutations produce significant modifications of both nuclear architecture and chromatin condensation." @default.
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• W1972602940 date "2003-11-01" @default.
• W1972602940 modified "2023-09-24" @default.
• W1972602940 title "A structural characterization of in situ chromatin on cell lines isolated from patients affected by ataxia telangiectasia" @default.
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• W1972602940 doi "https://doi.org/10.1016/s0141-8130(03)00060-6" @default.
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