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- W1972611601 abstract "Seabuckthorn (Hippophae rhamnoides L.) is a dioecious plant with berries containing high amounts of several bioactive compounds with nutritional and medicinal traits. It is also planted to control soil erosion. A genetic transformation procedure will facilitate studies of the control of plant development and interactions with symbionts and pathogens, and will provide a tool for plant breeding. Here, we present a particle bombardment method for transforming seabuckthorn. The early stages of induced adventitious shoots from roots were chosen as a novel target tissue for the transformation procedure. The root system was bombarded with gold particles coated with plasmid pRT99gus containing genes for plant kanamycin resistance and for β-glucuronidase expression, and shoots were regenerated under kanamycin selection. PCR analysis of the regenerated transformed lines confirmed the presence of a 603 bp gus (uidA) gene fragment and a 1.5 kb fragment from the 35S promoter in three shoots from independent transformation events." @default.
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- W1972611601 date "2014-01-01" @default.
- W1972611601 modified "2023-10-02" @default.
- W1972611601 title "Development of a Genetic Transformation Method for Seabuckthorn (<i>Hippophae rhamnoides</i> L.)" @default.
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- W1972611601 doi "https://doi.org/10.4236/ajps.2014.55067" @default.
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