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• W1972616012 abstract "To establish a stable and efficient immobilization technique under microwave irradiation, a focused microwave reaction system was used, where the temperature was set appropriately in the microwave system and cooling module to produce consecutive microwave irradiation. 2-Deoxy-D-ribose-5-phosphate aldolase (DERA) was rapidly and efficiently immobilized in mesocellular siliceous foams (MCFs) under microwave irradiation. When the output power in the microwave system was set to 30 W, after 3 min, 88.4% of the enzyme protein was coupled to the wall of the support pores and the specific activity of the immobilized enzyme was 2.24 U mg(-1), 149.2% higher than that of the free enzyme and 157.0% higher than that of the non-microwave-assisted immobilized enzyme. In catalysis, microwave-assisted immobilized DERA tolerated a wider range of both pH and temperature than other DERA preparations. The thermal and storage stabilities were also significantly improved. This focused; microwave-assisted immobilization technique has proven to be simple, stable and highly efficient. This technique could also be applied to other enzyme immobilizations." @default.
• W1972616012 created "2016-06-24" @default.
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• W1972616012 date "2011-01-01" @default.
• W1972616012 modified "2023-10-18" @default.
• W1972616012 title "Stable and efficient immobilization technique of aldolase under consecutive microwave irradiation at low temperature" @default.
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• W1972616012 doi "https://doi.org/10.1016/j.biortech.2010.08.048" @default.
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