Matches in SemOpenAlex for { <https://semopenalex.org/work/W1972622034> ?p ?o ?g. }
- W1972622034 abstract "Proceedings: AACR 104th Annual Meeting 2013; Apr 6-10, 2013; Washington, DCBackground. Pancreatic adenocarcinoma is a highly lethal disease, which is usually diagnosed in advanced stages for which there are little or no effective therapies. It has the worst prognosis of any major malignancy (3% 5-year survival) and is the fourth most common cause of cancer death yearly in the United States. Recently there have been several reports that the presence of cancer stem cells (CSCs) was related to the high refractoriness in pancreatic cancer. CSCs have two characteristics, self-renewal and differentiation, for maintenance of cancer tissues (Nature, 2001). Given that chemotherapies target generally against differentiated non-CSCs, small populations of CSCs survive and the recurrence of tumor growth will occur. Nevertheless, the underlined mechanism, which links between chemoresistance and stemness, remains to be elucidated. Considering that microRNAs are number-limited, functional nucleotides, those should be beneficial for efficient diagnostic and therapeutic approaches of pancreatic cancer.Matherials and Methods. Pancreatic cancer Panc1 cells were cultured in the medium containing increasing amounts of gemcitabine (GEM) to obtain several GEM-resistant clones. To identify the microRNAs, which play a critical role in the chemoresistance in pancreatic CSCs, we performed the serum-free floating culture of embryoid body-like structures by quick re-aggregation method (SFEBq), as a tool for separating CSCs; The SFEBq is the method for culture and concentration of neural stem cells, which can concentrate CSCs successfully in several types of cancer. The chemoresistance was studied by the MTT assay. RNAs were extracted, the genome-wide transcriptome was identified and networks were studied between stemness and chemoresistance.Results. The study of original Panc1 cells and four representative, independent GEM-resistant clones (R1, R2, R3 and R4) allowed the identification of CSC-specific and GEM-specific signal transduction pathways. The data were verified among four resistant clones. By utilizing specific inhibitors including small-molecule compounds and anti-microRNAs, we studied the network cross-talking CSCs and chemoresistance. Even if the SFEBq-enriched CSC clones were not exposed to GEM, their similarity to chemo-specific pathway reinforces that the small populations of CSCs would pre-exist as an innate resistance before treatment in patients. Such cross-talking pathway would be eligible to sensitize pancreatic cancer to chemotherapy, and to eradicate tumors.Conclusion. The data demonstrate the successful molecular dissection of stemness and chemoresistant pathways, and the present study provides the rationale for novel therapeutic approaches such as modified nucleotide medicines to target the pre-existing CSCs.Citation Format: Shinichiro Hasegawa, Hideshi Ishii, Shimpei Nishikawa, Hidetoshi Eguchi, Shogo Kobayashi, Hiroshi Wada, Naoki Hama, Hirofumi Akita, Koichi Kawamoto, Masamitsu Konno, Hisataka Ogawa, Katsuya Ohta, Yoshihiro Kano, Takahito Fukusumi, Atsushi Hamabe, Takenori Nishimura, Kunihiko Hinohara, Taroh Satoh, Noriko Gotoh, Yuichiro Doki, Masaki Mori, Hiroaki Nagano. Identification of microRNA networks in pancreatic cancer stem cells. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 4897. doi:10.1158/1538-7445.AM2013-4897" @default.
- W1972622034 created "2016-06-24" @default.
- W1972622034 creator A5007399417 @default.
- W1972622034 creator A5013781849 @default.
- W1972622034 creator A5014332130 @default.
- W1972622034 creator A5016421869 @default.
- W1972622034 creator A5017963746 @default.
- W1972622034 creator A5021500931 @default.
- W1972622034 creator A5024148200 @default.
- W1972622034 creator A5028227099 @default.
- W1972622034 creator A5028284770 @default.
- W1972622034 creator A5049927425 @default.
- W1972622034 creator A5058524907 @default.
- W1972622034 creator A5064829603 @default.
- W1972622034 creator A5065529459 @default.
- W1972622034 creator A5067647817 @default.
- W1972622034 creator A5068445312 @default.
- W1972622034 creator A5068569188 @default.
- W1972622034 creator A5069195280 @default.
- W1972622034 creator A5069574401 @default.
- W1972622034 creator A5073472318 @default.
- W1972622034 creator A5083677685 @default.
- W1972622034 creator A5085007436 @default.
- W1972622034 creator A5087070725 @default.
- W1972622034 date "2013-04-15" @default.
- W1972622034 modified "2023-09-26" @default.
- W1972622034 title "Abstract 4897: Identification of microRNA networks in pancreatic cancer stem cells." @default.
- W1972622034 doi "https://doi.org/10.1158/1538-7445.am2013-4897" @default.
- W1972622034 hasPublicationYear "2013" @default.
- W1972622034 type Work @default.
- W1972622034 sameAs 1972622034 @default.
- W1972622034 citedByCount "0" @default.
- W1972622034 crossrefType "proceedings-article" @default.
- W1972622034 hasAuthorship W1972622034A5007399417 @default.
- W1972622034 hasAuthorship W1972622034A5013781849 @default.
- W1972622034 hasAuthorship W1972622034A5014332130 @default.
- W1972622034 hasAuthorship W1972622034A5016421869 @default.
- W1972622034 hasAuthorship W1972622034A5017963746 @default.
- W1972622034 hasAuthorship W1972622034A5021500931 @default.
- W1972622034 hasAuthorship W1972622034A5024148200 @default.
- W1972622034 hasAuthorship W1972622034A5028227099 @default.
- W1972622034 hasAuthorship W1972622034A5028284770 @default.
- W1972622034 hasAuthorship W1972622034A5049927425 @default.
- W1972622034 hasAuthorship W1972622034A5058524907 @default.
- W1972622034 hasAuthorship W1972622034A5064829603 @default.
- W1972622034 hasAuthorship W1972622034A5065529459 @default.
- W1972622034 hasAuthorship W1972622034A5067647817 @default.
- W1972622034 hasAuthorship W1972622034A5068445312 @default.
- W1972622034 hasAuthorship W1972622034A5068569188 @default.
- W1972622034 hasAuthorship W1972622034A5069195280 @default.
- W1972622034 hasAuthorship W1972622034A5069574401 @default.
- W1972622034 hasAuthorship W1972622034A5073472318 @default.
- W1972622034 hasAuthorship W1972622034A5083677685 @default.
- W1972622034 hasAuthorship W1972622034A5085007436 @default.
- W1972622034 hasAuthorship W1972622034A5087070725 @default.
- W1972622034 hasConcept C104317684 @default.
- W1972622034 hasConcept C121608353 @default.
- W1972622034 hasConcept C126322002 @default.
- W1972622034 hasConcept C143998085 @default.
- W1972622034 hasConcept C145059251 @default.
- W1972622034 hasConcept C2779399171 @default.
- W1972622034 hasConcept C2780210213 @default.
- W1972622034 hasConcept C2780258809 @default.
- W1972622034 hasConcept C28328180 @default.
- W1972622034 hasConcept C502942594 @default.
- W1972622034 hasConcept C54355233 @default.
- W1972622034 hasConcept C55427017 @default.
- W1972622034 hasConcept C71924100 @default.
- W1972622034 hasConcept C86803240 @default.
- W1972622034 hasConcept C95444343 @default.
- W1972622034 hasConceptScore W1972622034C104317684 @default.
- W1972622034 hasConceptScore W1972622034C121608353 @default.
- W1972622034 hasConceptScore W1972622034C126322002 @default.
- W1972622034 hasConceptScore W1972622034C143998085 @default.
- W1972622034 hasConceptScore W1972622034C145059251 @default.
- W1972622034 hasConceptScore W1972622034C2779399171 @default.
- W1972622034 hasConceptScore W1972622034C2780210213 @default.
- W1972622034 hasConceptScore W1972622034C2780258809 @default.
- W1972622034 hasConceptScore W1972622034C28328180 @default.
- W1972622034 hasConceptScore W1972622034C502942594 @default.
- W1972622034 hasConceptScore W1972622034C54355233 @default.
- W1972622034 hasConceptScore W1972622034C55427017 @default.
- W1972622034 hasConceptScore W1972622034C71924100 @default.
- W1972622034 hasConceptScore W1972622034C86803240 @default.
- W1972622034 hasConceptScore W1972622034C95444343 @default.
- W1972622034 hasLocation W19726220341 @default.
- W1972622034 hasOpenAccess W1972622034 @default.
- W1972622034 hasPrimaryLocation W19726220341 @default.
- W1972622034 hasRelatedWork W1553388398 @default.
- W1972622034 hasRelatedWork W1564188857 @default.
- W1972622034 hasRelatedWork W1854105605 @default.
- W1972622034 hasRelatedWork W1967979352 @default.
- W1972622034 hasRelatedWork W1982536823 @default.
- W1972622034 hasRelatedWork W2025546862 @default.
- W1972622034 hasRelatedWork W2055070058 @default.
- W1972622034 hasRelatedWork W2068175976 @default.
- W1972622034 hasRelatedWork W2083032260 @default.
- W1972622034 hasRelatedWork W2091859485 @default.
- W1972622034 hasRelatedWork W2161727062 @default.
- W1972622034 hasRelatedWork W2319144496 @default.