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• W1972632145 endingPage "225" @default.
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• W1972632145 abstract "The gene encoding glycoprotein B of human cytomegalovirus (CMV) strain Towne was cloned, sequenced, and expressed in order to study potential targets for viral neutralization. Secondary structure analysis of the 907 amino acid protein predicted a 24 amino acid N-terminal signal sequence and a potential transmembrane region composed of two domains, 34 and 21 amino acids. The CMV (Towne) gB gene had a 94% nucleotide similarity and a 95% amino acid similarity to the CMV (AD169) gB gene [as described by M.P. Cranage et al. (1986, EMBO J. 5, 3057-3063)]. Transcriptional analysis of the CMV (Towne) gB coding strand revealed that the gB message (3.9 kb), was transcribed from this region as early as 4 hr postinfection, and well in advance of gB protein synthesis. Full-length and truncated versions of the gB gene were expressed in COS cells using expression vectors where transcription was driven by the SV40 early promoter or the CMV major immediate early promoter. Expression was detected by immunofluorescence and ELISA using the virus neutralizing murine monoclonal antibody 15D8 (L. Rasmussen, J. Mullenax, R. Nelson, and T.C. Merigan, 1985, J. Virol. 55, 274-280). This antibody had been shown previously to recognize a 55-kDa CMV virion protein and a related 130-kDa intracellular precursor. Amino acid sequence analysis of the N-terminus of the 55-kDa viral glycoprotein (gp55) showed that gp55 is derived from gB (gp130) by proteolytic cleavage and represents the C-terminal region of gp130. The truncated version of gB expressed in COS and CHO cells was also processed by proteolytic cleavage as demonstrated by Western blotting. Our study localizes the epitope recognized by 15D8 to within a 186 amino acid fragment of the gp55 protein. These results indicate that CMV gB is a target for neutralization and establishes gp55 as a candidate component for use in a subunit vaccine." @default.
• W1972632145 created "2016-06-24" @default.
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• W1972632145 date "1988-11-01" @default.
• W1972632145 modified "2023-09-27" @default.
• W1972632145 title "Human cytomegalovirus strain towne glycoprotein B is processed by proteolytic cleavage" @default.
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• W1972632145 cites W1517724708 @default.
• W1972632145 cites W1528710182 @default.
• W1972632145 cites W1531940905 @default.
• W1972632145 cites W1544551975 @default.
• W1972632145 cites W1559142908 @default.
• W1972632145 cites W1560109929 @default.
• W1972632145 cites W1592287063 @default.
• W1972632145 cites W1600871831 @default.
• W1972632145 cites W1743073606 @default.
• W1972632145 cites W1780875019 @default.
• W1972632145 cites W1825920775 @default.
• W1972632145 cites W1897816881 @default.
• W1972632145 cites W1932052686 @default.
• W1972632145 cites W1934602117 @default.
• W1972632145 cites W1947577878 @default.
• W1972632145 cites W1966683236 @default.
• W1972632145 cites W1966870518 @default.
• W1972632145 cites W1975304761 @default.
• W1972632145 cites W1979343286 @default.
• W1972632145 cites W1980464074 @default.
• W1972632145 cites W1984412128 @default.
• W1972632145 cites W1999899106 @default.
• W1972632145 cites W2004200027 @default.
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• W1972632145 cites W2023125347 @default.
• W1972632145 cites W2024994724 @default.
• W1972632145 cites W2026086486 @default.
• W1972632145 cites W2035799009 @default.
• W1972632145 cites W2036577722 @default.
• W1972632145 cites W2037199854 @default.
• W1972632145 cites W2040358152 @default.
• W1972632145 cites W2044780149 @default.
• W1972632145 cites W2047377191 @default.
• W1972632145 cites W2047472099 @default.
• W1972632145 cites W2053540318 @default.
• W1972632145 cites W2060333964 @default.
• W1972632145 cites W2063466305 @default.
• W1972632145 cites W2065766799 @default.
• W1972632145 cites W2066471744 @default.
• W1972632145 cites W2066635520 @default.
• W1972632145 cites W2071147879 @default.
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• W1972632145 cites W2081095625 @default.
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• W1972632145 cites W2089015567 @default.
• W1972632145 cites W2095450147 @default.
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• W1972632145 cites W2121542842 @default.
• W1972632145 cites W2124003901 @default.
• W1972632145 cites W2149736282 @default.
• W1972632145 cites W2151732089 @default.
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• W1972632145 doi "https://doi.org/10.1016/0042-6822(88)90071-2" @default.
• W1972632145 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2460994" @default.
• W1972632145 hasPublicationYear "1988" @default.
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