FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1972642171> ?p ?o ?g. }

• W1972642171 endingPage "194" @default.
• W1972642171 startingPage "184" @default.
• W1972642171 abstract "The Na+,K(+)-ATPase alpha 1, alpha 2, and alpha 3 subunit isoforms have been shown to be differentially expressed in the nonpigmented (NPE) and pigmented (PE) cells of the ocular ciliary epithelium (CE) (Martin-Vasallo et al., J. Cell. Physiol., 141:243-252, 1989; Ghosh et al., J. Biol. Chem., 265:2935-2940, 1990). In this study we analyzed and compared the pattern of expression of the multiple Na+,K(+)-ATPase alpha (alpha 1, alpha 2, alpha 3) subunit genes with the pattern of expression of the Na+,K(+)-ATPase beta (beta 1, beta 2) subunit genes along the bovine CE. We have selected three regions in the CE, referred to as 1) the anterior region of the pars plicata, near the iris; 2) the middle region of the pars plicata; and 3) the posterior region of the pars plana, near the ora serrata. Using isoform-specific cDNA probes and antibodies for the Na+,K(+)-ATPase alpha 1, alpha 2, alpha 3, beta 1, and beta 2 subunits on Northern and Western blot analysis, we found that mRNA and polypeptides are expressed in all three CE regions with different abundance. The pattern of expression of alpha and beta isoforms detected along the NPE cell layers suggests a gradient of alpha 1, alpha 2, alpha 3, beta 1, and beta 2 mRNAs and polypeptides that correlates with decreasing Na+,K(+)-ATPase activity from the most anterior region at the pars plicata towards the posterior region at the ora serrata. We also found marked differences in the pattern of immunolocalization of Na+,K(+)-ATPase alpha 1, alpha 2, alpha 3, beta 1, and beta 2 subunit isoforms in different regions of the CE. In the anterior region, NPE cells stained intensely at the basal lateral membrane with specific monoclonal and polyclonal antibodies for each of the alpha (alpha 1, alpha 2, alpha 3) and beta (beta 1, beta 2) Na,K-ATPase isoforms. In the middle and posterior regions of the CE, NPE cells showed lower or absent levels of staining with alpha 1, alpha 2, alpha 3, and beta 1 antibodies, although staining with beta 2 was abundant. In contrast, PE cells throughout the CE were stained at the basal lateral membrane by antibodies to alpha 1 and beta 1, while no staining signals were detected with the rest of the antibodies (i.e. alpha 2, alpha 3, and beta 2). Our results support the conclusion that the three alpha and two beta isoforms of the Na+,K(+)-ATPase are differentially expressed in the two cell layers that make up the CE.(ABSTRACT TRUNCATED AT 250 WORDS)" @default.
• W1972642171 created "2016-06-24" @default.
• W1972642171 creator A5013436965 @default.
• W1972642171 creator A5024057820 @default.
• W1972642171 creator A5040009127 @default.
• W1972642171 creator A5042232924 @default.
• W1972642171 creator A5062831547 @default.
• W1972642171 date "1991-11-01" @default.
• W1972642171 modified "2023-10-18" @default.
• W1972642171 title "Expression of multiple Na⁺,K⁺-ATPase genes reveals a gradient of isoforms along the nonpigmented ciliary epithelium: Functional implications in aqueous humor secretion" @default.
• W1972642171 cites W1497322979 @default.
• W1972642171 cites W1523864517 @default.
• W1972642171 cites W1571456015 @default.
• W1972642171 cites W1573051141 @default.
• W1972642171 cites W1604450112 @default.
• W1972642171 cites W1640540908 @default.
• W1972642171 cites W1775749144 @default.
• W1972642171 cites W1967705425 @default.
• W1972642171 cites W1977304490 @default.
• W1972642171 cites W1978018924 @default.
• W1972642171 cites W1998345338 @default.
• W1972642171 cites W2010586297 @default.
• W1972642171 cites W2011982929 @default.
• W1972642171 cites W2014669590 @default.
• W1972642171 cites W2043577188 @default.
• W1972642171 cites W2058113050 @default.
• W1972642171 cites W2058743162 @default.
• W1972642171 cites W2075227398 @default.
• W1972642171 cites W2078425162 @default.
• W1972642171 cites W2093166155 @default.
• W1972642171 cites W2100837269 @default.
• W1972642171 cites W2107540632 @default.
• W1972642171 cites W2110565876 @default.
• W1972642171 cites W2477710436 @default.
• W1972642171 cites W4294216491 @default.
• W1972642171 doi "https://doi.org/10.1002/jcp.1041490203" @default.
• W1972642171 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1660898" @default.
• W1972642171 hasPublicationYear "1991" @default.
• W1972642171 type Work @default.
• W1972642171 sameAs 1972642171 @default.
• W1972642171 citedByCount "61" @default.
• W1972642171 countsByYear W19726421712013 @default.
• W1972642171 countsByYear W19726421712014 @default.
• W1972642171 countsByYear W19726421712015 @default.
• W1972642171 countsByYear W19726421712018 @default.
• W1972642171 countsByYear W19726421712020 @default.
• W1972642171 countsByYear W19726421712021 @default.
• W1972642171 countsByYear W19726421712022 @default.
• W1972642171 countsByYear W19726421712023 @default.
• W1972642171 crossrefType "journal-article" @default.
• W1972642171 hasAuthorship W1972642171A5013436965 @default.
• W1972642171 hasAuthorship W1972642171A5024057820 @default.
• W1972642171 hasAuthorship W1972642171A5040009127 @default.
• W1972642171 hasAuthorship W1972642171A5042232924 @default.
• W1972642171 hasAuthorship W1972642171A5062831547 @default.
• W1972642171 hasConcept C104292427 @default.
• W1972642171 hasConcept C104317684 @default.
• W1972642171 hasConcept C150194340 @default.
• W1972642171 hasConcept C153911025 @default.
• W1972642171 hasConcept C159110408 @default.
• W1972642171 hasConcept C169760540 @default.
• W1972642171 hasConcept C177707886 @default.
• W1972642171 hasConcept C199360897 @default.
• W1972642171 hasConcept C2775944032 @default.
• W1972642171 hasConcept C2776174256 @default.
• W1972642171 hasConcept C2776415932 @default.
• W1972642171 hasConcept C2778434524 @default.
• W1972642171 hasConcept C2779105803 @default.
• W1972642171 hasConcept C41008148 @default.
• W1972642171 hasConcept C49453240 @default.
• W1972642171 hasConcept C53345823 @default.
• W1972642171 hasConcept C55493867 @default.
• W1972642171 hasConcept C64943373 @default.
• W1972642171 hasConcept C71924100 @default.
• W1972642171 hasConcept C83730767 @default.
• W1972642171 hasConcept C86803240 @default.
• W1972642171 hasConceptScore W1972642171C104292427 @default.
• W1972642171 hasConceptScore W1972642171C104317684 @default.
• W1972642171 hasConceptScore W1972642171C150194340 @default.
• W1972642171 hasConceptScore W1972642171C153911025 @default.
• W1972642171 hasConceptScore W1972642171C159110408 @default.
• W1972642171 hasConceptScore W1972642171C169760540 @default.
• W1972642171 hasConceptScore W1972642171C177707886 @default.
• W1972642171 hasConceptScore W1972642171C199360897 @default.
• W1972642171 hasConceptScore W1972642171C2775944032 @default.
• W1972642171 hasConceptScore W1972642171C2776174256 @default.
• W1972642171 hasConceptScore W1972642171C2776415932 @default.
• W1972642171 hasConceptScore W1972642171C2778434524 @default.
• W1972642171 hasConceptScore W1972642171C2779105803 @default.
• W1972642171 hasConceptScore W1972642171C41008148 @default.
• W1972642171 hasConceptScore W1972642171C49453240 @default.
• W1972642171 hasConceptScore W1972642171C53345823 @default.
• W1972642171 hasConceptScore W1972642171C55493867 @default.
• W1972642171 hasConceptScore W1972642171C64943373 @default.
• W1972642171 hasConceptScore W1972642171C71924100 @default.
• W1972642171 hasConceptScore W1972642171C83730767 @default.
• W1972642171 hasConceptScore W1972642171C86803240 @default.
• W1972642171 hasIssue "2" @default.

• next