FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1972742444> ?p ?o ?g. }

• W1972742444 endingPage "428" @default.
• W1972742444 startingPage "419" @default.
• W1972742444 abstract "Neisseria meningitides is a gram-negative diplococcus bacterium and is the main causative agent of meningitis and other meningococcal diseases. Alanine aminopeptidase from N. meningitides (NmAPN) belongs to the family of metallo-exopeptidase enzymes, which catalyze the removal of amino acids from the N-terminus of peptides and proteins, and are found among all the kingdoms of life. NmAPN is suggested to be mostly responsible for proteolysis and nutrition delivery, similar to the orthologs from other bacteria. To explore the possibility of NmAPN being a potential drug target for inhibition and development of novel therapeutic agents, the specificity of the S1 and S1' binding sites was explored using an integrated approach. Initially, an extensive library consisting of almost 100 fluorogenic substrates derived from both natural and unnatural amino acids, were used to obtain a detailed substrate fingerprint of the S1 pocket of NmAPN. A broad substrate tolerance of NmAPN was revealed, with bulky basic and hydrophobic ligands being the most favored substrates. Additionally, the potency of a set of organophosphorus inhibitors of neutral aminopeptidases, amino acid and dipeptide analogs was determined. Inhibition constants in the nanomolar range, determined for phosphinic dipeptides, proves the positive increase in inhibition impact of the P1' ligand elongation. The results were further verified via molecular modeling and docking of canonical aminopeptidase phosphinic dipeptide inhibitors in the NmAPN active site. These studies present comprehensive characterization of interactions responsible for specific ligand binding. This knowledge provides invaluable insight into understanding of the enzyme and development of novel NmAPN inhibitors." @default.
• W1972742444 created "2016-06-24" @default.
• W1972742444 creator A5000997540 @default.
• W1972742444 creator A5011576853 @default.
• W1972742444 creator A5014013198 @default.
• W1972742444 creator A5015893701 @default.
• W1972742444 creator A5018909606 @default.
• W1972742444 creator A5025897808 @default.
• W1972742444 creator A5061135367 @default.
• W1972742444 creator A5063684833 @default.
• W1972742444 creator A5063868367 @default.
• W1972742444 date "2013-02-01" @default.
• W1972742444 modified "2023-10-10" @default.
• W1972742444 title "An integrated approach to the ligand binding specificity of Neisseria meningitidis M1 alanine aminopeptidase by fluorogenic substrate profiling, inhibitory studies and molecular modeling" @default.
• W1972742444 cites W1964139478 @default.
• W1972742444 cites W1968567870 @default.
• W1972742444 cites W1974404575 @default.
• W1972742444 cites W1983342808 @default.
• W1972742444 cites W1989011168 @default.
• W1972742444 cites W1994631366 @default.
• W1972742444 cites W2004590340 @default.
• W1972742444 cites W2008932788 @default.
• W1972742444 cites W2017871159 @default.
• W1972742444 cites W2020780403 @default.
• W1972742444 cites W2048504001 @default.
• W1972742444 cites W2048521920 @default.
• W1972742444 cites W2048617934 @default.
• W1972742444 cites W206172689 @default.
• W1972742444 cites W2076675620 @default.
• W1972742444 cites W2095112833 @default.
• W1972742444 cites W2108581833 @default.
• W1972742444 cites W2110463636 @default.
• W1972742444 cites W2112662264 @default.
• W1972742444 cites W2128704535 @default.
• W1972742444 cites W2134606611 @default.
• W1972742444 cites W2138493390 @default.
• W1972742444 cites W2148890544 @default.
• W1972742444 cites W2153108266 @default.
• W1972742444 cites W2158635619 @default.
• W1972742444 cites W2158715838 @default.
• W1972742444 cites W2160782025 @default.
• W1972742444 cites W2166344811 @default.
• W1972742444 cites W2168298007 @default.
• W1972742444 cites W2180404361 @default.
• W1972742444 cites W2316005451 @default.
• W1972742444 cites W2316834908 @default.
• W1972742444 cites W2318066473 @default.
• W1972742444 cites W2324125498 @default.
• W1972742444 cites W2912985375 @default.
• W1972742444 cites W2953362575 @default.
• W1972742444 cites W4243726548 @default.
• W1972742444 doi "https://doi.org/10.1016/j.biochi.2012.10.018" @default.
• W1972742444 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3557594" @default.
• W1972742444 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23131591" @default.
• W1972742444 hasPublicationYear "2013" @default.
• W1972742444 type Work @default.
• W1972742444 sameAs 1972742444 @default.
• W1972742444 citedByCount "20" @default.
• W1972742444 countsByYear W19727424442012 @default.
• W1972742444 countsByYear W19727424442013 @default.
• W1972742444 countsByYear W19727424442014 @default.
• W1972742444 countsByYear W19727424442015 @default.
• W1972742444 countsByYear W19727424442016 @default.
• W1972742444 countsByYear W19727424442017 @default.
• W1972742444 countsByYear W19727424442019 @default.
• W1972742444 countsByYear W19727424442020 @default.
• W1972742444 countsByYear W19727424442021 @default.
• W1972742444 countsByYear W19727424442022 @default.
• W1972742444 countsByYear W19727424442023 @default.
• W1972742444 crossrefType "journal-article" @default.
• W1972742444 hasAuthorship W1972742444A5000997540 @default.
• W1972742444 hasAuthorship W1972742444A5011576853 @default.
• W1972742444 hasAuthorship W1972742444A5014013198 @default.
• W1972742444 hasAuthorship W1972742444A5015893701 @default.
• W1972742444 hasAuthorship W1972742444A5018909606 @default.
• W1972742444 hasAuthorship W1972742444A5025897808 @default.
• W1972742444 hasAuthorship W1972742444A5061135367 @default.
• W1972742444 hasAuthorship W1972742444A5063684833 @default.
• W1972742444 hasAuthorship W1972742444A5063868367 @default.
• W1972742444 hasBestOaLocation W19727424442 @default.
• W1972742444 hasConcept C116569031 @default.
• W1972742444 hasConcept C159110408 @default.
• W1972742444 hasConcept C170493617 @default.
• W1972742444 hasConcept C181199279 @default.
• W1972742444 hasConcept C185592680 @default.
• W1972742444 hasConcept C2776580952 @default.
• W1972742444 hasConcept C2779138802 @default.
• W1972742444 hasConcept C2779856020 @default.
• W1972742444 hasConcept C2780985610 @default.
• W1972742444 hasConcept C41183919 @default.
• W1972742444 hasConcept C41685203 @default.
• W1972742444 hasConcept C515207424 @default.
• W1972742444 hasConcept C54374919 @default.
• W1972742444 hasConcept C55493867 @default.
• W1972742444 hasConcept C71240020 @default.
• W1972742444 hasConcept C71924100 @default.
• W1972742444 hasConcept C86803240 @default.
• W1972742444 hasConceptScore W1972742444C116569031 @default.

• next