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- W1972794920 abstract "The morphology of Monascus cells was controlled and pigment fermentations scaled up from a 5 l jar to a 300 l pilot vessel. At a rotational speed of 350 rpm or less, serious mycelial aggregation was present with pigment yields of approximately 37.5 OD units. Monascus cells with long hairy mycelia were induced, resulting in viscous cultures. As the rotational speed increased from 350 to 700 rpm, the kLa values increased gradually from 0.003 to 0.029 s−1. A maximum pigment yield of 220 OD units was obtained at 500 rpm where mycelia with short branches were induced. The specific oxygen uptake rates (qO2) at 600 and 700 rpm were also lower than the rate at 500 rpm. Mycelia had short branches and appeared to be damaged by mechanical shear forces due to high agitation speeds. When seed cultures were grown in jar fermenters under high shear stress, the pigment yields and DOT values of fermentations were also high. Fermentations were successfully scaled up from 5 l jars to a 300 l pilot vessel based on a constant impeller speed of 12,500 cm min−1, which was the best condition for pigment production and cell morphology." @default.
- W1972794920 created "2016-06-24" @default.
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- W1972794920 date "2002-12-01" @default.
- W1972794920 modified "2023-10-01" @default.
- W1972794920 title "Morphology control of Monascus cells and scale-up of pigment fermentation" @default.
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- W1972794920 doi "https://doi.org/10.1016/s0032-9592(02)00095-x" @default.
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