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- W1972854403 abstract "Recently, several optogenetic tools have been developed to control the biochemical activity of signaling proteins with fast temporal and subcellular spatial resolutions. Among this tools, the cryptochrome CRY2 was probed to interact with its partner, CIBN, upon blue illumination. It was then showed that this light inducible dimerizer could be used to induce signaling perturbation in cells. An important issue with the use of these new dimerizers to dissect interacting protein networks is the mapping between the light input and the induced signaling perturbation. What should be the pattern of light to shine on cell to obtain a required distribution of active proteins?We combined TIRF microscopy with FRAP illumination to locally activate CRY2 in the cytoplasm while monitoring its recruitment to the basal plasma membrane (its partner, CIBN, being anchored to the membrane). With a simple biophysical analysis of diffusion and trapping processes grounded on experimentally measured parameters, we are able to propose simple set of rules to achieve a desired spatiotemporal distribution on the plasma membrane. In particular we showed that it is possible to establish a stable steady gradient even if the dissociation of the CRY2/CIBN complex is not light controllable. We then exploit this light induced dimerizer to control the activity of RhoGTPases with a fine spatial and temporal resolution." @default.
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- W1972854403 date "2013-01-01" @default.
- W1972854403 modified "2023-09-29" @default.
- W1972854403 title "Spatiotemporal Control of Light Induced Dimerizers" @default.
- W1972854403 doi "https://doi.org/10.1016/j.bpj.2012.11.3743" @default.
- W1972854403 hasPublicationYear "2013" @default.
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