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- W1972912682 endingPage "e29591" @default.
- W1972912682 startingPage "e29591" @default.
- W1972912682 abstract "Gene vectors derived from DNA transposable elements have become powerful molecular tools in biomedical research and are slowly moving into the clinic as carriers of therapeutic genes. Conventional uses of DNA transposon-based gene vehicles rely on the intracellular production of the transposase protein from transfected nucleic acids. The transposase mediates mobilization of the DNA transposon, which is typically provided in the context of plasmid DNA. In recent work, we established lentiviral protein transduction from Gag precursors as a new strategy for direct delivery of the transposase protein. Inspired by the natural properties of infecting viruses to carry their own enzymes, we loaded lentivirus-derived particles not only with vector genomes carrying the DNA transposon vector but also with hundreds of transposase subunits. Such particles were found to drive efficient transposition of the piggyBac transposable element in a range of different cell types, including primary cells, and offer a new transposase delivery approach that guarantees short-term activity and limits potential cytotoxicity. DNA transposon vectors, originally developed and launched as a non-viral alternative to viral integrating vectors, have truly become viral. Here, we briefly review our findings and speculate on the perspectives and potential advantages of transposase delivery by lentiviral protein transduction." @default.
- W1972912682 created "2016-06-24" @default.
- W1972912682 creator A5042892492 @default.
- W1972912682 creator A5082099200 @default.
- W1972912682 date "2014-05-01" @default.
- W1972912682 modified "2023-10-17" @default.
- W1972912682 title "Driving DNA transposition by lentiviral protein transduction" @default.
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- W1972912682 doi "https://doi.org/10.4161/mge.29591" @default.
- W1972912682 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4092313" @default.
- W1972912682 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25057443" @default.
- W1972912682 hasPublicationYear "2014" @default.
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