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• W1972962841 abstract "We have developed a coupled in vitro transcription–polyadenylation system to investigate RNA polymerase II (Pol II) termination, which depends on active polyadenylation of the nascent RNA. Specific G-rich sequences originally identified as binding sites for the transcription factor MAZ both pause Pol II and activate polyadenylation of an upstream poly(A) signal. They do not affect polyadenylation efficiency in an uncoupled cleavage assay. In contrast, pausing of Pol II elongation induced by a high-affinity DNA-binding protein does not activate polyadenylation, indicating that G-rich MAZ sequences have a specific effect on polyadenylation. They also promote intrinsic pausing of purified Pol II, indicating a general role in the modulation of cotranscriptional RNA processing events." @default.
• W1972962841 created "2016-06-24" @default.
• W1972962841 creator A5028313004 @default.
• W1972962841 creator A5088910266 @default.
• W1972962841 date "1999-05-01" @default.
• W1972962841 modified "2023-09-25" @default.
• W1972962841 title "Specific Transcriptional Pausing Activates Polyadenylation in a Coupled In Vitro System" @default.
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• W1972962841 doi "https://doi.org/10.1016/s1097-2765(00)80352-4" @default.
• W1972962841 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10360175" @default.
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