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• W1973001520 abstract "The effects of 5alpha-dihydrotestosterone-receptor complexes on transcription in human hypertrophied prostate tissue were studied in a cell-free system reconstituted from the various subcellular fractions prepared from specimens of the diseased gland. Two major RNA polymerase species were isolated from human hypertrophied prostate. These were designated A and B and were distinguishable by their preference for divalent cations and their sensitivity to salt and alpha-amanitin. Moreover, RNA polymerase B, but not RNA polymerase A, could effectively transcribe a prostate chromatin template. Any enzyme activity endogenous to some chromatin preparations was shown to be characteristic of RNA polymerase B. 5alpha-Dihydrotestoterone-receptor complexes were transferred into prostatic chromatin both steroid- and tissue-specifically. The association of steroid-receptor complexes with chromatin produced changes in template activity and increased the transcription of the chromatin by exogenous and endogenous RNA polymerase B. With a number of specimens, however, there was considerable variation in accessible cytoplasmic receptor sites, uptake of steroid-receptor complexes by chromatin preparations, the template activity of the chromatin and its response to steroid-receptor stimulation. Nevertheless, the transcription characteristics of human hypertrophied prostatic chromatin appear to be influenced by steroid-receptor complexes, and the extent of the response to added complexes would undoubtedly be governed by pre-existing complexes having had an earlier effect." @default.
• W1973001520 created "2016-06-24" @default.
• W1973001520 creator A5033211182 @default.
• W1973001520 creator A5080527709 @default.
• W1973001520 date "1976-08-01" @default.
• W1973001520 modified "2023-10-18" @default.
• W1973001520 title "Influence of steroid-receptor complexes on transcription by human hypertrophied prostatic RNA polymerases" @default.
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• W1973001520 doi "https://doi.org/10.1016/0303-7207(76)90089-7" @default.
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