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- W1973002261 abstract "The multicolour genomic in situ hybridization (mcGISH) method was improved in order to visualize the U b and M b genomes of Aegilops biuncialis Vis. (2n=4x=28, U b U b M b M b ). Hybridization probes prepared from the diploid U and M genome donors, Ae. umbellulata and Ae. comosa , resulted in clear hybridization signals on the U and M chromosomes in Ae. biuncialis . The random primed labelling method made it possible to decrease the blocking ratio to 1:30. McGISH allowed the simultaneous discrimination of individual Ae. biuncialis genomes and wheat chromosomes in γ-irradiated Triticum aestivum-Ae. biuncialis amphiploids (2n=70; AABBDDU b U b M b M b ). Dicentric chromosomes, terminal and interstitial translocations and centric fusions were detected in the irradiated generation. The irradiation-induced wheat- Ae. biuncialis intergenomic translocations will facilitate the successful introgression of useful agronomic traits into bread wheat." @default.
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- W1973002261 date "2010-09-01" @default.
- W1973002261 modified "2023-10-16" @default.
- W1973002261 title "Visualization of U and M genome chromosomes by multicolour genomic in situ hybridization in Aegilops biuncialis and Triticum aestivum-Ae. biuncialis amphiploids" @default.
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- W1973002261 doi "https://doi.org/10.1556/aagr.58.2010.3.1" @default.
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