FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1973002597> ?p ?o ?g. }

• W1973002597 endingPage "11260" @default.
• W1973002597 startingPage "11252" @default.
• W1973002597 abstract "The Escherichia coli twin-arginine translocation (Tat) system transports fully folded and assembled proteins across the inner membrane into the periplasmic space. Traditionally, in vitro protein translocation studies have been performed using gel-based transport assays. This technique suffers from low time resolution, and often, an inability to distinguish between different steps in a continuously occurring translocation process. To address these limitations, we have developed an in vitro FRET-based assay that reports on an early step in the Tat translocation process in real-time. The natural Tat substrate pre-SufI was labeled with Alexa532 (donor), and the fluorescent protein mCherry (acceptor) was fused to the C terminus of TatB or TatC. The colored Tat proteins were easily visible during purification, enabling identification of a highly active inverted membrane vesicle (IMV) fraction yielding transport rates with NADH almost an order of magnitude faster than previously reported. When pre-SufI was bound to the translocon, FRET was observed for both Tat proteins. FRET was diminished upon addition of nonfluorescent pre-SufI, indicating that the initial binding step is reversible. When the membranes were energized with NADH, the FRET signal was lost after a short delay. These data suggest a model in which a Tat cargo initially associates with the TatBC complex, and an electric field gradient is required for the cargo to proceed to the next stage of transport. This cargo migration away from the TatBC complex requires a significant fraction of the total transport time." @default.
• W1973002597 created "2016-06-24" @default.
• W1973002597 creator A5042858180 @default.
• W1973002597 creator A5075907326 @default.
• W1973002597 creator A5085962625 @default.
• W1973002597 date "2012-03-01" @default.
• W1973002597 modified "2023-10-03" @default.
• W1973002597 title "Kinetics of Precursor Interactions with the Bacterial Tat Translocase Detected by Real-time FRET" @default.
• W1973002597 cites W1557173992 @default.
• W1973002597 cites W1604304986 @default.
• W1973002597 cites W1873199501 @default.
• W1973002597 cites W1935158889 @default.
• W1973002597 cites W1965314886 @default.
• W1973002597 cites W1974202616 @default.
• W1973002597 cites W1975615339 @default.
• W1973002597 cites W1979490144 @default.
• W1973002597 cites W1981002074 @default.
• W1973002597 cites W1981397867 @default.
• W1973002597 cites W1982527241 @default.
• W1973002597 cites W1985205346 @default.
• W1973002597 cites W1988357511 @default.
• W1973002597 cites W1999207123 @default.
• W1973002597 cites W2006175108 @default.
• W1973002597 cites W2008293899 @default.
• W1973002597 cites W2010816372 @default.
• W1973002597 cites W2027685467 @default.
• W1973002597 cites W2028219441 @default.
• W1973002597 cites W2028622989 @default.
• W1973002597 cites W2028819914 @default.
• W1973002597 cites W2052470499 @default.
• W1973002597 cites W2057796783 @default.
• W1973002597 cites W2076876639 @default.
• W1973002597 cites W2083021096 @default.
• W1973002597 cites W2085614442 @default.
• W1973002597 cites W2085779944 @default.
• W1973002597 cites W2097634886 @default.
• W1973002597 cites W2104543109 @default.
• W1973002597 cites W2109050242 @default.
• W1973002597 cites W2115734610 @default.
• W1973002597 cites W2116666954 @default.
• W1973002597 cites W2121839643 @default.
• W1973002597 cites W2124444587 @default.
• W1973002597 cites W2130394977 @default.
• W1973002597 cites W2132728702 @default.
• W1973002597 cites W2133542385 @default.
• W1973002597 cites W2135227852 @default.
• W1973002597 cites W2139198526 @default.
• W1973002597 cites W2143599839 @default.
• W1973002597 cites W2149781820 @default.
• W1973002597 cites W2154936481 @default.
• W1973002597 cites W2159071226 @default.
• W1973002597 doi "https://doi.org/10.1074/jbc.m111.324525" @default.
• W1973002597 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3322843" @default.
• W1973002597 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22315217" @default.
• W1973002597 hasPublicationYear "2012" @default.
• W1973002597 type Work @default.
• W1973002597 sameAs 1973002597 @default.
• W1973002597 citedByCount "29" @default.
• W1973002597 countsByYear W19730025972012 @default.
• W1973002597 countsByYear W19730025972013 @default.
• W1973002597 countsByYear W19730025972014 @default.
• W1973002597 countsByYear W19730025972015 @default.
• W1973002597 countsByYear W19730025972016 @default.
• W1973002597 countsByYear W19730025972017 @default.
• W1973002597 countsByYear W19730025972018 @default.
• W1973002597 countsByYear W19730025972019 @default.
• W1973002597 countsByYear W19730025972021 @default.
• W1973002597 countsByYear W19730025972022 @default.
• W1973002597 crossrefType "journal-article" @default.
• W1973002597 hasAuthorship W1973002597A5042858180 @default.
• W1973002597 hasAuthorship W1973002597A5075907326 @default.
• W1973002597 hasAuthorship W1973002597A5085962625 @default.
• W1973002597 hasBestOaLocation W19730025971 @default.
• W1973002597 hasConcept C104317684 @default.
• W1973002597 hasConcept C10858879 @default.
• W1973002597 hasConcept C113035283 @default.
• W1973002597 hasConcept C115335371 @default.
• W1973002597 hasConcept C121332964 @default.
• W1973002597 hasConcept C12554922 @default.
• W1973002597 hasConcept C138626823 @default.
• W1973002597 hasConcept C142613039 @default.
• W1973002597 hasConcept C144647389 @default.
• W1973002597 hasConcept C167625842 @default.
• W1973002597 hasConcept C185592680 @default.
• W1973002597 hasConcept C199229279 @default.
• W1973002597 hasConcept C201663137 @default.
• W1973002597 hasConcept C2779260011 @default.
• W1973002597 hasConcept C33292294 @default.
• W1973002597 hasConcept C41625074 @default.
• W1973002597 hasConcept C547475151 @default.
• W1973002597 hasConcept C55493867 @default.
• W1973002597 hasConcept C57581600 @default.
• W1973002597 hasConcept C62520636 @default.
• W1973002597 hasConcept C86803240 @default.
• W1973002597 hasConcept C91881484 @default.
• W1973002597 hasConcept C96305047 @default.
• W1973002597 hasConceptScore W1973002597C104317684 @default.
• W1973002597 hasConceptScore W1973002597C10858879 @default.

• next