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- W1973009957 abstract "Abstract The induction or ornithine decarboxylase activity ( l -ornithine carboxy-lyase, EC 4.1.1.17) by either 8-bromoadenosine 3′,5′ cyclic monophosphate (8Br-cAMP) or the tumor promoting phorbol ester, 12- O -tetradecanoyl-13-acetate (TPA) was studied in a series of Chinese hamster ovary (CHO) cell mutants deficient in the enzyme cAMP-dependent protein kinase (ATP:protein phosphotransferase, EC 2.7.1.37). 8Br-cAMP induced ornithine decarboxylase activity in the parental or wild-type cell line (10001) in a dose-dependent manner, with 1 mM 8Br-cAMP causing a 10–12-fold increase in ornithine decarboxylase activity, which was maximal at 2–3 h following addition of the analog. Similar treatment with 1 mM 8Br-cAMP caused only a 0–3-fold increase in ornithine decarboxylase activity in four cAMP-dependent protein kinase mutants studied (10215, 10248, 10260, 10265), indicating that functional cAMP-dependent protein kinase is required for the 8Br-cAMP induced increase in ornithine decarboxylase activity. The tumor promoting phorbol ester, TPA (1.6 μM), led to a 10–15-fold increase in ornithine decarboxylase activity in the parental cell line (10001) with maximal levels reached at 3–4 h following addition of the agent. In three out of four cAMP-dependent protein kinase mutants, 1.6 μM TPA caused an 8–10-fold increase in ornithine decarboxylase activity, demonstrating that TPA caused an elevation in ornithine decarboxylase activity by a mechanism not totally dependent upon a functional cAMP-dependent protein kinase system. Both 8Br-cAMP and TPA were capable of stimulating the accumulation of putrescine (the product of ornithine decarboxylase) in the wild-type cell line (10001) at 6 h following treatment. However, only TPA was capable of stimulating an accumulation of putrescine in the cAMP-dependent protein kinase mutants. Neither 8Br-cAMP nor TPA increased the level of spermidine or spermine at 6, 10 or 24 h following treatment in either the parental cell line or cAMP-dependent protein kinase mutants." @default.
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- W1973009957 date "1983-04-01" @default.
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- W1973009957 title "Cyclic AMP and tumor promoters cause differential induction of ornithine decarboxylase and accumulation of putrescine in Chinese hamster ovary cells deficient in cyclic AMP-dependent protein kinase" @default.
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- W1973009957 doi "https://doi.org/10.1016/0167-4889(83)90070-8" @default.
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