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- W1973012987 abstract "The potential of building multi-cytotoxic T lymphocyte (CTL) epitope antigens in combination with the nucleic acid immunization technology is explored for development of acquired immunodeficiency syndrome (AIDS) and malaria vaccines. A novel minimal vector pTH for direct gene transfer was constructed for efficient expression of vaccine antigens and used as a vehicle for human immunodeficiency virus (HIV)- and Plasmodium falciparum-derived polyepitope genes. Two murine epitopes were included into these constructs to allow for testing of vaccine immunogenicity in small animals. The results showed that a single DNA injection generated CTL responses in all 15 vaccinated mice. The elicited CTL precursor frequencies were estimated in an interferon-γ (IFN-γ)-based ELISPOT assay and found to be an average of 300 (range 4–1346) peptide-responding cells per 106 splenocytes." @default.
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- W1973012987 date "1998-02-01" @default.
- W1973012987 modified "2023-10-16" @default.
- W1973012987 title "DNA multi-CTL epitope vaccines for HIV and Plasmodium falciparum: immunogenicity in mice" @default.
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- W1973012987 doi "https://doi.org/10.1016/s0264-410x(97)00296-x" @default.
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