FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1973014079> ?p ?o ?g. }

• W1973014079 endingPage "28" @default.
• W1973014079 startingPage "119" @default.
• W1973014079 abstract "We have studied the interaction between P1 and P2 purinoceptors in purified type-1 astrocyte cultures from postnatal days 7-8 rat cerebella using single cell microfluorimetry with fura-2. The stimulation of astrocytes with ATP elicits rapid [Ca2+]i transients showing an EC50 value of 7.9 +/- 0.3 microM. Costimulation of type-1 astrocytes with adenosine and ineffective ATP concentrations (0.1 or 1 microM) evoked [Ca2+]i transients that correspond to 60% of the maximal ATP response. NECA (5'-N-ethylcarboxamidoadenosine) was the only agonist that mimicked the adenosine effect and showed an EC50 value of 0.17 +/- 0.01 microM. This value was identical to that obtained for the cAMP production stimulation, indicating that A2B receptors coupled to adenylate cyclase activation were involved. The presence of A2B adenosine receptors was also confirmed by immocytochemistry experiments. When astrocytes were costimulated with isoproterenol and ineffective ATP concentrations similar [Ca2+]i transients were observed. The treatment of astrocytes with cholera toxin potentiated ATP calcium signals, lowering the EC50 value for ATP to 1.5 +/- 0.2 microM. However, the pretreatment of cells with forskolin or a permeable cAMP analogue had no effect on ATP calcium responses. These results indicated that the potentiation mechanism was elicited before the adenylate cyclase activation. We could conclude that in type-1 astrocytes, the activation of A2B adenosine receptors or other signals positively coupled to adenylate cyclase stimulation strongly potentiate metabotropic calcium responses to ATP. The potentiation was parallel but independent on cAMP accumulation suggesting the involvement of beta gamma subunits released after Gs stimulation." @default.
• W1973014079 created "2016-06-24" @default.
• W1973014079 creator A5003375625 @default.
• W1973014079 creator A5043879414 @default.
• W1973014079 creator A5044519676 @default.
• W1973014079 creator A5070082098 @default.
• W1973014079 creator A5076013134 @default.
• W1973014079 creator A5085751554 @default.
• W1973014079 date "1999-04-01" @default.
• W1973014079 modified "2023-10-17" @default.
• W1973014079 title "Potentiation of ATP calcium responses by A2B receptor stimulation and other signals coupled to Gs proteins in type-1 cerebellar astrocytes." @default.
• W1973014079 cites W1535611266 @default.
• W1973014079 cites W1545688394 @default.
• W1973014079 cites W1549940137 @default.
• W1973014079 cites W1922582391 @default.
• W1973014079 cites W1967739047 @default.
• W1973014079 cites W1969761928 @default.
• W1973014079 cites W1979541885 @default.
• W1973014079 cites W1982553763 @default.
• W1973014079 cites W1982888042 @default.
• W1973014079 cites W1988461354 @default.
• W1973014079 cites W1991864133 @default.
• W1973014079 cites W1998401125 @default.
• W1973014079 cites W2007206888 @default.
• W1973014079 cites W2011676677 @default.
• W1973014079 cites W2015763227 @default.
• W1973014079 cites W2025242478 @default.
• W1973014079 cites W2026398072 @default.
• W1973014079 cites W2028011576 @default.
• W1973014079 cites W2039493042 @default.
• W1973014079 cites W2045067703 @default.
• W1973014079 cites W2045743982 @default.
• W1973014079 cites W2046903291 @default.
• W1973014079 cites W2053686834 @default.
• W1973014079 cites W2059994807 @default.
• W1973014079 cites W2065009203 @default.
• W1973014079 cites W2068685899 @default.
• W1973014079 cites W2069578625 @default.
• W1973014079 cites W2071489012 @default.
• W1973014079 cites W2079265669 @default.
• W1973014079 cites W2079440796 @default.
• W1973014079 cites W2080129938 @default.
• W1973014079 cites W2084606850 @default.
• W1973014079 cites W2095502686 @default.
• W1973014079 cites W2102747751 @default.
• W1973014079 cites W2108408639 @default.
• W1973014079 cites W2115588325 @default.
• W1973014079 cites W2122832621 @default.
• W1973014079 cites W2141260882 @default.
• W1973014079 cites W2160262187 @default.
• W1973014079 cites W2169518110 @default.
• W1973014079 cites W2170860372 @default.
• W1973014079 cites W4236341500 @default.
• W1973014079 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10384877" @default.
• W1973014079 hasPublicationYear "1999" @default.
• W1973014079 type Work @default.
• W1973014079 sameAs 1973014079 @default.
• W1973014079 citedByCount "34" @default.
• W1973014079 countsByYear W19730140792012 @default.
• W1973014079 countsByYear W19730140792013 @default.
• W1973014079 countsByYear W19730140792014 @default.
• W1973014079 countsByYear W19730140792015 @default.
• W1973014079 countsByYear W19730140792016 @default.
• W1973014079 countsByYear W19730140792017 @default.
• W1973014079 countsByYear W19730140792018 @default.
• W1973014079 countsByYear W19730140792019 @default.
• W1973014079 countsByYear W19730140792022 @default.
• W1973014079 crossrefType "journal-article" @default.
• W1973014079 hasAuthorship W1973014079A5003375625 @default.
• W1973014079 hasAuthorship W1973014079A5043879414 @default.
• W1973014079 hasAuthorship W1973014079A5044519676 @default.
• W1973014079 hasAuthorship W1973014079A5070082098 @default.
• W1973014079 hasAuthorship W1973014079A5076013134 @default.
• W1973014079 hasAuthorship W1973014079A5085751554 @default.
• W1973014079 hasConcept C126322002 @default.
• W1973014079 hasConcept C134018914 @default.
• W1973014079 hasConcept C170493617 @default.
• W1973014079 hasConcept C180526460 @default.
• W1973014079 hasConcept C24998067 @default.
• W1973014079 hasConcept C25274449 @default.
• W1973014079 hasConcept C26702167 @default.
• W1973014079 hasConcept C2776991684 @default.
• W1973014079 hasConcept C2777542381 @default.
• W1973014079 hasConcept C2778938600 @default.
• W1973014079 hasConcept C2779276759 @default.
• W1973014079 hasConcept C2780069284 @default.
• W1973014079 hasConcept C529278444 @default.
• W1973014079 hasConcept C55493867 @default.
• W1973014079 hasConcept C67907053 @default.
• W1973014079 hasConcept C71924100 @default.
• W1973014079 hasConcept C86803240 @default.
• W1973014079 hasConceptScore W1973014079C126322002 @default.
• W1973014079 hasConceptScore W1973014079C134018914 @default.
• W1973014079 hasConceptScore W1973014079C170493617 @default.
• W1973014079 hasConceptScore W1973014079C180526460 @default.
• W1973014079 hasConceptScore W1973014079C24998067 @default.
• W1973014079 hasConceptScore W1973014079C25274449 @default.
• W1973014079 hasConceptScore W1973014079C26702167 @default.

• next