Matches in SemOpenAlex for { <https://semopenalex.org/work/W1973048105> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W1973048105 endingPage "206" @default.
- W1973048105 startingPage "200" @default.
- W1973048105 abstract "The C4 mesophyll-chloroplast stromal enzyme pyruvate,orthophosphate dikinase (PPDK) regulatory protein (RP) catalyzes the activation/dephosphorylation and inactivation/phosphorylation of PPDK. This bifunctional converter enzyme has been partially purified from maize leaf extracts approximately 100-fold to a final specific PPDK-inactivation activity of 3-10 units PPDK/min/mg protein and an overall recovery of 1-5%. Purification of active RP was accomplished by (NH4)2SO4 fractionation and sequential column chromatography on dye-ligand and size-exclusion or FPLC-based anion-exchange matrices. Immunoblot analyses of size-exclusion and anion-exchange fractionated preparations indicate that NADP-malate dehydrogenase (MDH) and ribulose-bisphosphate carboxylase/oxygenase are major contaminants, respectively. During sequential dye-ligand and anion-exchange chromatography, RP activity copurifies with a ∼48-kDa polypeptide on silver-stained denaturing gels. Both RP activities are readily detected in maize mesophyll-chloroplast stromal extracts, but unlike stromal PPDK and NADP-MDH, the putative, ∼48-kDa RP polypeptide is barely detectable in silver-stained gels. Our collective data indicate that RP constitutes <0.04% of the total soluble maize-leaf protein and <1% of its target enzyme, contrary to a previous report. PPDK-inactivation and -activation activities are quantitatively equivalent in light- and dark-adapted maize-leaf tissue rapidly extracted and assayed at either pH 7.0 or 8.0, suggesting that the opposing RP activities are not regulated differentially by reversible covalent modification or pH effects." @default.
- W1973048105 created "2016-06-24" @default.
- W1973048105 creator A5028325882 @default.
- W1973048105 creator A5070949945 @default.
- W1973048105 creator A5083913566 @default.
- W1973048105 date "1994-01-01" @default.
- W1973048105 modified "2023-09-23" @default.
- W1973048105 title "Partial Purification and Characterization of Maize-Leaf Pyruvate, Orthophosphate Dikinase Regulatory Protein: A Low-Abundance, Mesophyll-Chloroplast Stromal Protein" @default.
- W1973048105 cites W1978909890 @default.
- W1973048105 cites W1991819883 @default.
- W1973048105 cites W1992677231 @default.
- W1973048105 cites W1993277768 @default.
- W1973048105 cites W2076337455 @default.
- W1973048105 cites W2095323370 @default.
- W1973048105 doi "https://doi.org/10.1006/abbi.1994.1028" @default.
- W1973048105 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8311453" @default.
- W1973048105 hasPublicationYear "1994" @default.
- W1973048105 type Work @default.
- W1973048105 sameAs 1973048105 @default.
- W1973048105 citedByCount "26" @default.
- W1973048105 countsByYear W19730481052017 @default.
- W1973048105 countsByYear W19730481052018 @default.
- W1973048105 crossrefType "journal-article" @default.
- W1973048105 hasAuthorship W1973048105A5028325882 @default.
- W1973048105 hasAuthorship W1973048105A5070949945 @default.
- W1973048105 hasAuthorship W1973048105A5083913566 @default.
- W1973048105 hasConcept C104317684 @default.
- W1973048105 hasConcept C116569031 @default.
- W1973048105 hasConcept C170493617 @default.
- W1973048105 hasConcept C179247698 @default.
- W1973048105 hasConcept C181199279 @default.
- W1973048105 hasConcept C185592680 @default.
- W1973048105 hasConcept C193363816 @default.
- W1973048105 hasConcept C195310446 @default.
- W1973048105 hasConcept C198684833 @default.
- W1973048105 hasConcept C206967267 @default.
- W1973048105 hasConcept C55493867 @default.
- W1973048105 hasConcept C69305403 @default.
- W1973048105 hasConceptScore W1973048105C104317684 @default.
- W1973048105 hasConceptScore W1973048105C116569031 @default.
- W1973048105 hasConceptScore W1973048105C170493617 @default.
- W1973048105 hasConceptScore W1973048105C179247698 @default.
- W1973048105 hasConceptScore W1973048105C181199279 @default.
- W1973048105 hasConceptScore W1973048105C185592680 @default.
- W1973048105 hasConceptScore W1973048105C193363816 @default.
- W1973048105 hasConceptScore W1973048105C195310446 @default.
- W1973048105 hasConceptScore W1973048105C198684833 @default.
- W1973048105 hasConceptScore W1973048105C206967267 @default.
- W1973048105 hasConceptScore W1973048105C55493867 @default.
- W1973048105 hasConceptScore W1973048105C69305403 @default.
- W1973048105 hasIssue "1" @default.
- W1973048105 hasLocation W19730481051 @default.
- W1973048105 hasLocation W19730481052 @default.
- W1973048105 hasOpenAccess W1973048105 @default.
- W1973048105 hasPrimaryLocation W19730481051 @default.
- W1973048105 hasRelatedWork W1966828512 @default.
- W1973048105 hasRelatedWork W1973048105 @default.
- W1973048105 hasRelatedWork W1976046259 @default.
- W1973048105 hasRelatedWork W1977313588 @default.
- W1973048105 hasRelatedWork W1992181775 @default.
- W1973048105 hasRelatedWork W2032513106 @default.
- W1973048105 hasRelatedWork W2070457453 @default.
- W1973048105 hasRelatedWork W2085869919 @default.
- W1973048105 hasRelatedWork W2350141476 @default.
- W1973048105 hasRelatedWork W2374065000 @default.
- W1973048105 hasVolume "308" @default.
- W1973048105 isParatext "false" @default.
- W1973048105 isRetracted "false" @default.
- W1973048105 magId "1973048105" @default.
- W1973048105 workType "article" @default.