FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1973053078> ?p ?o ?g. }

• W1973053078 endingPage "1239" @default.
• W1973053078 startingPage "1225" @default.
• W1973053078 abstract "The secondary structure and sequences influencing the expression and selection of the AUG initiator codon in the yeast Saccharomyces cerevisiae were investigated with two fused genes, which were composed of either the CYC7 or CYC1 leader regions, respectively, linked to the lacZ coding region. In addition, the strains contained the upf1-delta disruption, which stabilized mRNAs that had premature termination codons, resulting in wild-type levels. The following major conclusions were reached by measuring beta-galactosidase activities in yeast strains having integrated single copies of the fused genes with various alterations in the 89 and 38 nucleotide-long untranslated CYC7 and CYC1 leader regions, respectively. The leader region adjacent to the AUG initiator codon was dispensable, but the nucleotide preceding the AUG initiator at position -3 modified the efficiency of translation by less than twofold, exhibiting an order of preference A > G > C > U. Upstream out-of-frame AUG triplets diminished initiation at the normal site, from essentially complete inhibition to approximately 50% inhibition, depending on the position of the upstream AUG triplet and on the context (-3 position nucleotides) of the two AUG triplets. In this regard, complete inhibition occurred when the upstream and downstream AUG triplets were closer together, and when the upstream and downstream AUG triplets had, respectively, optimal and suboptimal contexts. Thus, leaky scanning occurs in yeast, similar to its occurrence in higher eukaryotes. In contrast, termination codons between two AUG triplets causes reinitiation at the downstream AUG in higher eukaryotes, but not generally in yeast. Our results and the results of others with GCN4 mRNA and its derivatives indicate that reinitiation is not a general phenomenon in yeast, and that special sequences are required." @default.
• W1973053078 created "2016-06-24" @default.
• W1973053078 creator A5018834567 @default.
• W1973053078 creator A5048234589 @default.
• W1973053078 creator A5058764175 @default.
• W1973053078 creator A5078623413 @default.
• W1973053078 date "1996-03-01" @default.
• W1973053078 modified "2023-10-10" @default.
• W1973053078 title "mRNA sequences influencing translation and the selection of AUG initiator codons in the yeast Saccharomyces cerevisiae" @default.
• W1973053078 cites W105923574 @default.
• W1973053078 cites W1549063736 @default.
• W1973053078 cites W1675134876 @default.
• W1973053078 cites W1679870850 @default.
• W1973053078 cites W1773106913 @default.
• W1973053078 cites W1821377433 @default.
• W1973053078 cites W1822797028 @default.
• W1973053078 cites W1891582045 @default.
• W1973053078 cites W1895421371 @default.
• W1973053078 cites W1950655336 @default.
• W1973053078 cites W1976105576 @default.
• W1973053078 cites W197886776 @default.
• W1973053078 cites W1980645073 @default.
• W1973053078 cites W1985202675 @default.
• W1973053078 cites W1992663641 @default.
• W1973053078 cites W1995737813 @default.
• W1973053078 cites W1999019973 @default.
• W1973053078 cites W2003217244 @default.
• W1973053078 cites W2003228141 @default.
• W1973053078 cites W2009045393 @default.
• W1973053078 cites W2012288774 @default.
• W1973053078 cites W2019025253 @default.
• W1973053078 cites W2020828649 @default.
• W1973053078 cites W2021289001 @default.
• W1973053078 cites W2021978074 @default.
• W1973053078 cites W2024366860 @default.
• W1973053078 cites W2025763720 @default.
• W1973053078 cites W2066825851 @default.
• W1973053078 cites W2072971665 @default.
• W1973053078 cites W2082546954 @default.
• W1973053078 cites W2084265940 @default.
• W1973053078 cites W2095935754 @default.
• W1973053078 cites W2110374270 @default.
• W1973053078 cites W2114991027 @default.
• W1973053078 cites W2134887033 @default.
• W1973053078 cites W2145557287 @default.
• W1973053078 cites W2147402219 @default.
• W1973053078 cites W2154128645 @default.
• W1973053078 cites W2155456813 @default.
• W1973053078 cites W2158017403 @default.
• W1973053078 cites W2163846244 @default.
• W1973053078 cites W2168847939 @default.
• W1973053078 cites W2171423621 @default.
• W1973053078 cites W2888511473 @default.
• W1973053078 cites W4237588029 @default.
• W1973053078 cites W4238877186 @default.
• W1973053078 cites W4240065971 @default.
• W1973053078 cites W4240922224 @default.
• W1973053078 cites W4252931792 @default.
• W1973053078 cites W4256140813 @default.
• W1973053078 doi "https://doi.org/10.1111/j.1365-2958.1996.tb02468.x" @default.
• W1973053078 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8730865" @default.
• W1973053078 hasPublicationYear "1996" @default.
• W1973053078 type Work @default.
• W1973053078 sameAs 1973053078 @default.
• W1973053078 citedByCount "58" @default.
• W1973053078 countsByYear W19730530782012 @default.
• W1973053078 countsByYear W19730530782013 @default.
• W1973053078 countsByYear W19730530782014 @default.
• W1973053078 countsByYear W19730530782016 @default.
• W1973053078 countsByYear W19730530782017 @default.
• W1973053078 countsByYear W19730530782019 @default.
• W1973053078 countsByYear W19730530782020 @default.
• W1973053078 countsByYear W19730530782021 @default.
• W1973053078 crossrefType "journal-article" @default.
• W1973053078 hasAuthorship W1973053078A5018834567 @default.
• W1973053078 hasAuthorship W1973053078A5048234589 @default.
• W1973053078 hasAuthorship W1973053078A5058764175 @default.
• W1973053078 hasAuthorship W1973053078A5078623413 @default.
• W1973053078 hasConcept C104317684 @default.
• W1973053078 hasConcept C105580179 @default.
• W1973053078 hasConcept C149364088 @default.
• W1973053078 hasConcept C151730666 @default.
• W1973053078 hasConcept C167625842 @default.
• W1973053078 hasConcept C176990463 @default.
• W1973053078 hasConcept C20580545 @default.
• W1973053078 hasConcept C2777576037 @default.
• W1973053078 hasConcept C2779222958 @default.
• W1973053078 hasConcept C2779343474 @default.
• W1973053078 hasConcept C4718897 @default.
• W1973053078 hasConcept C47289529 @default.
• W1973053078 hasConcept C512185932 @default.
• W1973053078 hasConcept C54355233 @default.
• W1973053078 hasConcept C86803240 @default.
• W1973053078 hasConcept C89604277 @default.
• W1973053078 hasConcept C91779695 @default.
• W1973053078 hasConceptScore W1973053078C104317684 @default.
• W1973053078 hasConceptScore W1973053078C105580179 @default.
• W1973053078 hasConceptScore W1973053078C149364088 @default.

• next