Matches in SemOpenAlex for { <https://semopenalex.org/work/W1973103398> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W1973103398 endingPage "8" @default.
- W1973103398 startingPage "1" @default.
- W1973103398 abstract "The molecular mechanisms controlling gene expression are still poorly understood in apicomplexan parasites. Here, we report the characterization of a homolog of the single strand binding proteins (named TgSsossB) in Toxoplasma gondii. We previously showed that TgSsossB interacts with the TgAlba proteins that are involved in translation regulation. We examined the role of TgSsossB in stress-mediated response, and particularly the role of its arginine–glycine–glycine (RGG) repeats domain. TgSsossB recombinant protein is able to bind to single strand DNA and RNA in a sequence-independent manner, but not to double stranded DNA. We showed that the RGG motif is not involved in this ability to bind to nucleic acid. We produced a mutant tagged strain lacking the RGG motif of TgSsossB using the knock-in strategy. We observed that this strain exhibited a fitness defect compared with the parental parasites. Moreover, the mutant strain produced fewer plaques in stress conditions, a defect that is due to a slow growth phenotype when extracellular parasites are exposed to stress. At the molecular level, we showed that the TgSsossB protein lacking a RGG motif lost its ability to interact with TgAlba2 and an isoform of TgAlba1, indicating that the TgAlba complex is likely non-functional in those parasites. Thus, our findings define the RGG domain of TgSsossB as a protein–protein interaction platform and underline the role of the TgAlba–TgSsossB complex in stress-mediated response." @default.
- W1973103398 created "2016-06-24" @default.
- W1973103398 creator A5002603079 @default.
- W1973103398 creator A5025126447 @default.
- W1973103398 creator A5033311057 @default.
- W1973103398 date "2014-08-01" @default.
- W1973103398 modified "2023-10-18" @default.
- W1973103398 title "A RGG motif protein is involved in Toxoplasma gondii stress-mediated response" @default.
- W1973103398 cites W1929629802 @default.
- W1973103398 cites W1969766619 @default.
- W1973103398 cites W1976150575 @default.
- W1973103398 cites W1982073960 @default.
- W1973103398 cites W1996446827 @default.
- W1973103398 cites W2037183555 @default.
- W1973103398 cites W2040417365 @default.
- W1973103398 cites W2060178110 @default.
- W1973103398 cites W2064596229 @default.
- W1973103398 cites W2066645277 @default.
- W1973103398 cites W2069092332 @default.
- W1973103398 cites W2137026987 @default.
- W1973103398 cites W2143687466 @default.
- W1973103398 cites W2146926661 @default.
- W1973103398 cites W2149676146 @default.
- W1973103398 cites W2153436893 @default.
- W1973103398 cites W2167790255 @default.
- W1973103398 cites W2171373236 @default.
- W1973103398 cites W2171810840 @default.
- W1973103398 doi "https://doi.org/10.1016/j.molbiopara.2014.07.009" @default.
- W1973103398 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25089036" @default.
- W1973103398 hasPublicationYear "2014" @default.
- W1973103398 type Work @default.
- W1973103398 sameAs 1973103398 @default.
- W1973103398 citedByCount "5" @default.
- W1973103398 countsByYear W19731033982016 @default.
- W1973103398 countsByYear W19731033982017 @default.
- W1973103398 countsByYear W19731033982018 @default.
- W1973103398 countsByYear W19731033982023 @default.
- W1973103398 crossrefType "journal-article" @default.
- W1973103398 hasAuthorship W1973103398A5002603079 @default.
- W1973103398 hasAuthorship W1973103398A5025126447 @default.
- W1973103398 hasAuthorship W1973103398A5033311057 @default.
- W1973103398 hasConcept C104317684 @default.
- W1973103398 hasConcept C105580179 @default.
- W1973103398 hasConcept C117745874 @default.
- W1973103398 hasConcept C117838200 @default.
- W1973103398 hasConcept C127716648 @default.
- W1973103398 hasConcept C132677234 @default.
- W1973103398 hasConcept C143065580 @default.
- W1973103398 hasConcept C149364088 @default.
- W1973103398 hasConcept C158730165 @default.
- W1973103398 hasConcept C41282012 @default.
- W1973103398 hasConcept C53345823 @default.
- W1973103398 hasConcept C54355233 @default.
- W1973103398 hasConcept C552990157 @default.
- W1973103398 hasConcept C55493867 @default.
- W1973103398 hasConcept C67705224 @default.
- W1973103398 hasConcept C86803240 @default.
- W1973103398 hasConcept C95444343 @default.
- W1973103398 hasConceptScore W1973103398C104317684 @default.
- W1973103398 hasConceptScore W1973103398C105580179 @default.
- W1973103398 hasConceptScore W1973103398C117745874 @default.
- W1973103398 hasConceptScore W1973103398C117838200 @default.
- W1973103398 hasConceptScore W1973103398C127716648 @default.
- W1973103398 hasConceptScore W1973103398C132677234 @default.
- W1973103398 hasConceptScore W1973103398C143065580 @default.
- W1973103398 hasConceptScore W1973103398C149364088 @default.
- W1973103398 hasConceptScore W1973103398C158730165 @default.
- W1973103398 hasConceptScore W1973103398C41282012 @default.
- W1973103398 hasConceptScore W1973103398C53345823 @default.
- W1973103398 hasConceptScore W1973103398C54355233 @default.
- W1973103398 hasConceptScore W1973103398C552990157 @default.
- W1973103398 hasConceptScore W1973103398C55493867 @default.
- W1973103398 hasConceptScore W1973103398C67705224 @default.
- W1973103398 hasConceptScore W1973103398C86803240 @default.
- W1973103398 hasConceptScore W1973103398C95444343 @default.
- W1973103398 hasFunder F4320320883 @default.
- W1973103398 hasFunder F4320322892 @default.
- W1973103398 hasIssue "1" @default.
- W1973103398 hasLocation W19731033981 @default.
- W1973103398 hasLocation W19731033982 @default.
- W1973103398 hasOpenAccess W1973103398 @default.
- W1973103398 hasPrimaryLocation W19731033981 @default.
- W1973103398 hasRelatedWork W1990902542 @default.
- W1973103398 hasRelatedWork W1993706132 @default.
- W1973103398 hasRelatedWork W2088525343 @default.
- W1973103398 hasRelatedWork W2254769804 @default.
- W1973103398 hasRelatedWork W2751343946 @default.
- W1973103398 hasRelatedWork W2808551098 @default.
- W1973103398 hasRelatedWork W2906196802 @default.
- W1973103398 hasRelatedWork W2950118990 @default.
- W1973103398 hasRelatedWork W2960668286 @default.
- W1973103398 hasRelatedWork W3165554045 @default.
- W1973103398 hasVolume "196" @default.
- W1973103398 isParatext "false" @default.
- W1973103398 isRetracted "false" @default.
- W1973103398 magId "1973103398" @default.
- W1973103398 workType "article" @default.