Matches in SemOpenAlex for { <https://semopenalex.org/work/W1973147092> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W1973147092 endingPage "263" @default.
- W1973147092 startingPage "259" @default.
- W1973147092 abstract "Background: Biotin is a water-soluble vitamin which plays an important biochemical role in a variety of carboxylase-mediated metabolic reactions. Determination of biotin status for the diagnosis of biotin deficiency is crucial. Methods: We describe a solid-phase protein-binding assay involving [ 125 I]iodostreptavidin as a tracer for the determination of biotin in plasma. The assay was conducted in one step by incubation of a fixed amount of [ 125 I]iodostreptavidin as binding reagent with varying amounts of biotin, diluted in biotin-free plasma (standard curve) or unknown samples in tubes previously coated with biotin linked to goat antirabbit IgG. Increasing amounts of biotin in the standard or unknown samples in tubes previously coated with biotin occupy more sites on iodostreptavidin, resulting in fewer counts bound to tubes. The effects of the incubation time and temperature on the competitive binding of biotin with iodostreptavidin were tested. Results: The detection limit of the plasma assay for biotin was 100 pmol/L. Only 100 µL of plasma were necessary for the assay, which was performed within 6 h. The dilutions of plasma and synthetic biotin gave a parallel response. Plasma biotin levels ranged from 0·49 to 1·33 nmol/L (mean 0·76 nmol/L) in healthy subjects. The intraand inter-assay coefficients of variation were 3·5% and 10%, respectively, at a concentration of 0·27 nmol/L. Conclusions: This assay was suitable for the direct measurement of biotin in human plasma and was robust and sensitive enough for screening for biotin deficiency." @default.
- W1973147092 created "2016-06-24" @default.
- W1973147092 creator A5015542912 @default.
- W1973147092 creator A5051966034 @default.
- W1973147092 date "2003-05-01" @default.
- W1973147092 modified "2023-09-25" @default.
- W1973147092 title "A sensitive and practical competitive radioassay for plasma biotin" @default.
- W1973147092 cites W1545161548 @default.
- W1973147092 cites W167749306 @default.
- W1973147092 cites W176513737 @default.
- W1973147092 cites W1893651774 @default.
- W1973147092 cites W1966214454 @default.
- W1973147092 cites W1991127274 @default.
- W1973147092 cites W2322460444 @default.
- W1973147092 doi "https://doi.org/10.1258/000456303321610574" @default.
- W1973147092 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12803839" @default.
- W1973147092 hasPublicationYear "2003" @default.
- W1973147092 type Work @default.
- W1973147092 sameAs 1973147092 @default.
- W1973147092 citedByCount "16" @default.
- W1973147092 countsByYear W19731470922016 @default.
- W1973147092 countsByYear W19731470922017 @default.
- W1973147092 countsByYear W19731470922019 @default.
- W1973147092 countsByYear W19731470922020 @default.
- W1973147092 countsByYear W19731470922023 @default.
- W1973147092 crossrefType "journal-article" @default.
- W1973147092 hasAuthorship W1973147092A5015542912 @default.
- W1973147092 hasAuthorship W1973147092A5051966034 @default.
- W1973147092 hasConcept C140137476 @default.
- W1973147092 hasConcept C142724271 @default.
- W1973147092 hasConcept C181199279 @default.
- W1973147092 hasConcept C184112429 @default.
- W1973147092 hasConcept C185592680 @default.
- W1973147092 hasConcept C204787440 @default.
- W1973147092 hasConcept C25642318 @default.
- W1973147092 hasConcept C2776294945 @default.
- W1973147092 hasConcept C2776940978 @default.
- W1973147092 hasConcept C2778204606 @default.
- W1973147092 hasConcept C43617362 @default.
- W1973147092 hasConcept C55493867 @default.
- W1973147092 hasConcept C71924100 @default.
- W1973147092 hasConceptScore W1973147092C140137476 @default.
- W1973147092 hasConceptScore W1973147092C142724271 @default.
- W1973147092 hasConceptScore W1973147092C181199279 @default.
- W1973147092 hasConceptScore W1973147092C184112429 @default.
- W1973147092 hasConceptScore W1973147092C185592680 @default.
- W1973147092 hasConceptScore W1973147092C204787440 @default.
- W1973147092 hasConceptScore W1973147092C25642318 @default.
- W1973147092 hasConceptScore W1973147092C2776294945 @default.
- W1973147092 hasConceptScore W1973147092C2776940978 @default.
- W1973147092 hasConceptScore W1973147092C2778204606 @default.
- W1973147092 hasConceptScore W1973147092C43617362 @default.
- W1973147092 hasConceptScore W1973147092C55493867 @default.
- W1973147092 hasConceptScore W1973147092C71924100 @default.
- W1973147092 hasIssue "3" @default.
- W1973147092 hasLocation W19731470921 @default.
- W1973147092 hasLocation W19731470922 @default.
- W1973147092 hasOpenAccess W1973147092 @default.
- W1973147092 hasPrimaryLocation W19731470921 @default.
- W1973147092 hasRelatedWork W1562086046 @default.
- W1973147092 hasRelatedWork W1973147092 @default.
- W1973147092 hasRelatedWork W1976951212 @default.
- W1973147092 hasRelatedWork W2031924560 @default.
- W1973147092 hasRelatedWork W2061541440 @default.
- W1973147092 hasRelatedWork W294245764 @default.
- W1973147092 hasRelatedWork W2971012003 @default.
- W1973147092 hasRelatedWork W301689074 @default.
- W1973147092 hasRelatedWork W339813795 @default.
- W1973147092 hasRelatedWork W350378836 @default.
- W1973147092 hasVolume "40" @default.
- W1973147092 isParatext "false" @default.
- W1973147092 isRetracted "false" @default.
- W1973147092 magId "1973147092" @default.
- W1973147092 workType "article" @default.