Matches in SemOpenAlex for { <https://semopenalex.org/work/W1973152912> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W1973152912 endingPage "430" @default.
- W1973152912 startingPage "422" @default.
- W1973152912 abstract "A triacylglycerol lipase (EC 3.1.1.3) was purified about 60-fold from rat liver cytosol by delipidation with acetone and ethyl ether, hydroxyapatite and Sephadex G-100 column chromatographies and isoelectrofocusing electrophoresis. The partially purified enzyme had a molecular weight of approximately 42 000 and an isoelectric point of 7.2. The Km for trioleylglycerol was 0.33 mM and the pH optimum was around 8.0. The activity of the enzyme was not dependent on serum lipoproteins, but was stimulated about 2-fold by several proteins such as serum albumin, lipoproteins, γ-globulin and ovalbumin. The lipase hydrolyzed trioleylglycerol to oleic acid and glycerol. NaCl had no effect on the enzymatic activity. Some physical and kinetic properties of the partially purified lipid-free lipase were different from those of crude non-delipidated lipase and also from those of a neutral triacylglycerol lipase which was recently purified partially from pig liver cytosol (Ledford, J.H. and Alaupovic, P. (1975) Biochim. Biophys. Acta 398, 132–148)." @default.
- W1973152912 created "2016-06-24" @default.
- W1973152912 creator A5000949448 @default.
- W1973152912 creator A5006534769 @default.
- W1973152912 creator A5012424340 @default.
- W1973152912 creator A5035422515 @default.
- W1973152912 date "1977-06-01" @default.
- W1973152912 modified "2023-10-17" @default.
- W1973152912 title "Partial purification and characterization of a triacylglycerol lipase from rat liver cytosol" @default.
- W1973152912 cites W1483442031 @default.
- W1973152912 cites W1511844964 @default.
- W1973152912 cites W1520348962 @default.
- W1973152912 cites W1527313809 @default.
- W1973152912 cites W1529819945 @default.
- W1973152912 cites W1565198178 @default.
- W1973152912 cites W1611482599 @default.
- W1973152912 cites W1625660792 @default.
- W1973152912 cites W1720895391 @default.
- W1973152912 cites W1750773183 @default.
- W1973152912 cites W1753210391 @default.
- W1973152912 cites W1775749144 @default.
- W1973152912 cites W1865276518 @default.
- W1973152912 cites W1969498369 @default.
- W1973152912 cites W1969954912 @default.
- W1973152912 cites W1997121496 @default.
- W1973152912 cites W2022743847 @default.
- W1973152912 cites W2045853510 @default.
- W1973152912 cites W2053018930 @default.
- W1973152912 cites W2068430810 @default.
- W1973152912 cites W2089794527 @default.
- W1973152912 cites W2095504155 @default.
- W1973152912 cites W2098268788 @default.
- W1973152912 cites W2137826815 @default.
- W1973152912 cites W2151374190 @default.
- W1973152912 cites W2158514594 @default.
- W1973152912 cites W2271700846 @default.
- W1973152912 cites W2321515281 @default.
- W1973152912 cites W2324350525 @default.
- W1973152912 cites W4301955712 @default.
- W1973152912 doi "https://doi.org/10.1016/0005-2760(77)90213-2" @default.
- W1973152912 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18201" @default.
- W1973152912 hasPublicationYear "1977" @default.
- W1973152912 type Work @default.
- W1973152912 sameAs 1973152912 @default.
- W1973152912 citedByCount "4" @default.
- W1973152912 crossrefType "journal-article" @default.
- W1973152912 hasAuthorship W1973152912A5000949448 @default.
- W1973152912 hasAuthorship W1973152912A5006534769 @default.
- W1973152912 hasAuthorship W1973152912A5012424340 @default.
- W1973152912 hasAuthorship W1973152912A5035422515 @default.
- W1973152912 hasConcept C163994747 @default.
- W1973152912 hasConcept C181199279 @default.
- W1973152912 hasConcept C185592680 @default.
- W1973152912 hasConcept C2776125364 @default.
- W1973152912 hasConcept C2779697368 @default.
- W1973152912 hasConcept C2780786110 @default.
- W1973152912 hasConcept C2781157690 @default.
- W1973152912 hasConcept C2781403372 @default.
- W1973152912 hasConcept C43617362 @default.
- W1973152912 hasConcept C50476208 @default.
- W1973152912 hasConcept C55493867 @default.
- W1973152912 hasConcept C94879076 @default.
- W1973152912 hasConcept C96294017 @default.
- W1973152912 hasConcept C98539663 @default.
- W1973152912 hasConceptScore W1973152912C163994747 @default.
- W1973152912 hasConceptScore W1973152912C181199279 @default.
- W1973152912 hasConceptScore W1973152912C185592680 @default.
- W1973152912 hasConceptScore W1973152912C2776125364 @default.
- W1973152912 hasConceptScore W1973152912C2779697368 @default.
- W1973152912 hasConceptScore W1973152912C2780786110 @default.
- W1973152912 hasConceptScore W1973152912C2781157690 @default.
- W1973152912 hasConceptScore W1973152912C2781403372 @default.
- W1973152912 hasConceptScore W1973152912C43617362 @default.
- W1973152912 hasConceptScore W1973152912C50476208 @default.
- W1973152912 hasConceptScore W1973152912C55493867 @default.
- W1973152912 hasConceptScore W1973152912C94879076 @default.
- W1973152912 hasConceptScore W1973152912C96294017 @default.
- W1973152912 hasConceptScore W1973152912C98539663 @default.
- W1973152912 hasIssue "3" @default.
- W1973152912 hasLocation W19731529121 @default.
- W1973152912 hasLocation W19731529122 @default.
- W1973152912 hasOpenAccess W1973152912 @default.
- W1973152912 hasPrimaryLocation W19731529121 @default.
- W1973152912 hasRelatedWork W1933918084 @default.
- W1973152912 hasRelatedWork W1973152912 @default.
- W1973152912 hasRelatedWork W2013676631 @default.
- W1973152912 hasRelatedWork W2035818010 @default.
- W1973152912 hasRelatedWork W2041275422 @default.
- W1973152912 hasRelatedWork W2051023003 @default.
- W1973152912 hasRelatedWork W2140358694 @default.
- W1973152912 hasRelatedWork W2157022344 @default.
- W1973152912 hasRelatedWork W2336579743 @default.
- W1973152912 hasRelatedWork W2414686794 @default.
- W1973152912 hasVolume "487" @default.
- W1973152912 isParatext "false" @default.
- W1973152912 isRetracted "false" @default.
- W1973152912 magId "1973152912" @default.
- W1973152912 workType "article" @default.