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- W1973153964 abstract "Synthesis of phospholipids and phospholipid fatty acids in isolated perfused rat lung was studied. The perfusion fluid was a Krebs-Ringer bicarbonate buffer containing a14C labeled substrate. It was found that 1-14C-acetate, 1-14C-laurate, 1-14C-palmitate, 1-14C-stearate, 1-14C-oleate, or U-14C-D-glucose was incorporated into tissue lipids in the isolated perfused lung at a rate geeater than that in incubated minced tissue. However, the patterns of the newly synthesized lipids from these two systems were similar. In 1 hr of perfusion, 6.8, 3, 14.5, 7.5, 7, and 2% of the initial14C-radioactivity of 1-14C-acetate, 1-14C-laurate, 1-14C-palmitate, 1-14C-stearate, 1-14C-oleate, and U-14C-D-glucose, respectively, were incorporated into phospholipids. Phospholipid fatty acids accounted for 95–96% total phospholipids-14C when14C-substrates, other than glucose, were used. For glucose, only 20% phospholipids-14C was in phospholipid fatty acids. More than 80% phospholipid fatty acids-14C was in palmitic acid when 1-14C-acetate and U-14C-D-glucose were used, while 37, 61, 80, and 94% phospholipid fatty acid-14C from 1-14C-laurate, 1-14C-sterate, 1-14C-oleate, and 1-14C-palmitate, respectively were recovered in the original form of the fatty acid used. The newly synthesized phospholipid fatty acid (13–24%) from 1-14C-laurate, 1-14C-stearate, and 1-14C-oleate was palmitic, and 10% of phospholipid fatty acid from 1-14C-stearate was in oleic acid. Hydrolysis by phospholipase A showed that14C from perfused substrates was esterified to both α and β positions of phospholipids. It was found that positional selectivity of phospholipid fatty acids was determined by chain length, degree of unsaturation, and source of fatty acid." @default.
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- W1973153964 date "1974-02-01" @default.
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- W1973153964 title "Synthesis of phospholipids and phospholipid fatty acids by isolated perfused rat lung" @default.
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- W1973153964 doi "https://doi.org/10.1007/bf02532126" @default.
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