Matches in SemOpenAlex for { <https://semopenalex.org/work/W1973156770> ?p ?o ?g. }
- W1973156770 endingPage "1158" @default.
- W1973156770 startingPage "1148" @default.
- W1973156770 abstract "In this study we demonstrated that neural rosettes derived from human ES cells can give rise either to neural crest precursors, following expansion in presence of bFGF and EGF, or to dopaminergic precursors after exposure to ventralizing factors Shh and FGF8. Both regionalised precursors are capable of extensive proliferation and differentiation towards the corresponding terminally differentiated cell types. In particular, peripheral neurons, cartilage, bone, smooth muscle cells and also pigmented cells were obtained from neural crest precursors while tyrosine hydroxylase and Nurr1 positive dopaminergic neurons were derived from FGF8 and Shh primed rosette cells. Gene expression and immunocytochemistry analyses confirmed the expression of dorsal and neural crest genes such as Sox10, Slug, p75, FoxD3, Pax7 in neural precursors from bFGF-EGF exposed rosettes. By contrast, priming of rosettes with FGF8 and Shh induced the expression of dopaminergic markers Engrailed1, Pax2, Pitx3, floor plate marker FoxA2 and radial glia markers Blbp and Glast, the latter in agreement with the origin of dopaminergic precursors from floor plate radial glia. Moreover, in vivo transplant of proliferating Shh/FGF8 primed precursors in parkinsonian rats demonstrated engraftment and terminal dopaminergic differentiation. In conclusion, we demonstrated the derivation of long-term self-renewing precursors of selected regional identity as potential cell reservoirs for cell therapy applications, such as CNS degenerative diseases, or for the development of toxicological tests." @default.
- W1973156770 created "2016-06-24" @default.
- W1973156770 creator A5007011035 @default.
- W1973156770 creator A5007019878 @default.
- W1973156770 creator A5025344954 @default.
- W1973156770 creator A5029800720 @default.
- W1973156770 creator A5032383705 @default.
- W1973156770 creator A5037128407 @default.
- W1973156770 creator A5055662303 @default.
- W1973156770 date "2010-04-01" @default.
- W1973156770 modified "2023-10-15" @default.
- W1973156770 title "Long-term culture and differentiation of CNS precursors derived from anterior human neural rosettes following exposure to ventralizing factors" @default.
- W1973156770 cites W1601656880 @default.
- W1973156770 cites W1970082173 @default.
- W1973156770 cites W1983274440 @default.
- W1973156770 cites W1988865085 @default.
- W1973156770 cites W1993909108 @default.
- W1973156770 cites W2005707140 @default.
- W1973156770 cites W2013393348 @default.
- W1973156770 cites W2018235103 @default.
- W1973156770 cites W2019558931 @default.
- W1973156770 cites W2022873912 @default.
- W1973156770 cites W2033284482 @default.
- W1973156770 cites W2034641989 @default.
- W1973156770 cites W2063503250 @default.
- W1973156770 cites W2064496329 @default.
- W1973156770 cites W2069811195 @default.
- W1973156770 cites W2071451454 @default.
- W1973156770 cites W2072837173 @default.
- W1973156770 cites W2074498796 @default.
- W1973156770 cites W2076892678 @default.
- W1973156770 cites W2081978832 @default.
- W1973156770 cites W2082893764 @default.
- W1973156770 cites W2083939751 @default.
- W1973156770 cites W2084412384 @default.
- W1973156770 cites W2105643617 @default.
- W1973156770 cites W2107499423 @default.
- W1973156770 cites W2111191613 @default.
- W1973156770 cites W2111525324 @default.
- W1973156770 cites W2122191105 @default.
- W1973156770 cites W2125908939 @default.
- W1973156770 cites W2144083195 @default.
- W1973156770 cites W2148633182 @default.
- W1973156770 cites W2152069828 @default.
- W1973156770 cites W2163987873 @default.
- W1973156770 cites W2165400025 @default.
- W1973156770 doi "https://doi.org/10.1016/j.yexcr.2010.02.013" @default.
- W1973156770 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20171210" @default.
- W1973156770 hasPublicationYear "2010" @default.
- W1973156770 type Work @default.
- W1973156770 sameAs 1973156770 @default.
- W1973156770 citedByCount "34" @default.
- W1973156770 countsByYear W19731567702012 @default.
- W1973156770 countsByYear W19731567702013 @default.
- W1973156770 countsByYear W19731567702014 @default.
- W1973156770 countsByYear W19731567702015 @default.
- W1973156770 countsByYear W19731567702016 @default.
- W1973156770 countsByYear W19731567702017 @default.
- W1973156770 countsByYear W19731567702018 @default.
- W1973156770 countsByYear W19731567702019 @default.
- W1973156770 countsByYear W19731567702020 @default.
- W1973156770 countsByYear W19731567702021 @default.
- W1973156770 countsByYear W19731567702022 @default.
- W1973156770 countsByYear W19731567702023 @default.
- W1973156770 crossrefType "journal-article" @default.
- W1973156770 hasAuthorship W1973156770A5007011035 @default.
- W1973156770 hasAuthorship W1973156770A5007019878 @default.
- W1973156770 hasAuthorship W1973156770A5025344954 @default.
- W1973156770 hasAuthorship W1973156770A5029800720 @default.
- W1973156770 hasAuthorship W1973156770A5032383705 @default.
- W1973156770 hasAuthorship W1973156770A5037128407 @default.
- W1973156770 hasAuthorship W1973156770A5055662303 @default.
- W1973156770 hasConcept C104317684 @default.
- W1973156770 hasConcept C124964019 @default.
- W1973156770 hasConcept C126749454 @default.
- W1973156770 hasConcept C137183658 @default.
- W1973156770 hasConcept C148738053 @default.
- W1973156770 hasConcept C1491633281 @default.
- W1973156770 hasConcept C169760540 @default.
- W1973156770 hasConcept C170493617 @default.
- W1973156770 hasConcept C196843134 @default.
- W1973156770 hasConcept C2776094402 @default.
- W1973156770 hasConcept C2780071072 @default.
- W1973156770 hasConcept C513476851 @default.
- W1973156770 hasConcept C55493867 @default.
- W1973156770 hasConcept C74373430 @default.
- W1973156770 hasConcept C78577261 @default.
- W1973156770 hasConcept C86803240 @default.
- W1973156770 hasConcept C95444343 @default.
- W1973156770 hasConceptScore W1973156770C104317684 @default.
- W1973156770 hasConceptScore W1973156770C124964019 @default.
- W1973156770 hasConceptScore W1973156770C126749454 @default.
- W1973156770 hasConceptScore W1973156770C137183658 @default.
- W1973156770 hasConceptScore W1973156770C148738053 @default.
- W1973156770 hasConceptScore W1973156770C1491633281 @default.
- W1973156770 hasConceptScore W1973156770C169760540 @default.
- W1973156770 hasConceptScore W1973156770C170493617 @default.
- W1973156770 hasConceptScore W1973156770C196843134 @default.