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- W1973180564 abstract "Rapid detection ofCampylobacter jejuniby PCR directly from foods, without prior growth steps, would be beneficial for the poultry industry. We have previously reported a PCR assay that allows detection of this bacterium after 48 h growth on Campy cefex agar. We have now developed a more rapid nested PCR assay that specifically detectsC. jejuniin chicken washes that have not undergone any lengthy growth steps prior to PCR. For the nested reaction, an external set of primers, C-1 and C-4, are used for 24 cycles. At this time, 1 μl of the PCR product is removed and added to a second reaction. The second PCR assay is run with C-1 and an internal primer, C-2, for 24 cycles. A single band on a 4% NuSieve agarose gel at 122 bp was apparent withC. jejunicells at a sensitivity of 102cfu ml−1. With this method chicken carcasses can be washed andC. jejuniidentified all within 1 day. We detectedC. jejuniin approximately 80% of four groups of chickens using this method. The identifications have been confirmed by standard microbiological techniques." @default.
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- W1973180564 date "1997-08-01" @default.
- W1973180564 modified "2023-10-16" @default.
- W1973180564 title "Rapid PCR with nested primers for direct detection ofCampylobacter jejuniin chicken washes" @default.
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- W1973180564 doi "https://doi.org/10.1006/mcpr.1997.0116" @default.
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