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- W1973219123 abstract "Besides gonadotropins various other peptide factors have been implicated in the ovarian follicular growth and steroidogenesis. In this study, the effect of insulin like growth factor-1 on caprine granulosa cell steroidogenesis was investigated in vitro using serum free culture medium. Caprine granulosa cells were obtained from antral follicles classified into three classes: small, <3 mm; medium, 3–6 mm and large, >6 mm in diameter. IGF-1 (10–500 ng/ml) stimulated the secretion of estradiol and progesterone by granulosa cells from all three categories of follicles. However, the effect was more profound on cells from large follicles. IGF-1 (10–500 ng/ml) acted in synergy with FSH (100 ng/ml) to stimulate steroidogenesis by granulosa cells from all three categories of follicles, again the effect was more pronounced in cells from larger follicles. In summary, IGF-1 at physiological concentrations significantly influenced the steroidogenic activity of caprine granulosa cells cultured under serum free conditions. The results also suggest that estrogen and progesterone biosynthesis is differentially regulated by IGF-1 depending upon the size of the follicles from which the cells were obtained." @default.
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- W1973219123 date "1999-07-01" @default.
- W1973219123 modified "2023-10-16" @default.
- W1973219123 title "Effect of recombinant insulin like growth factor-1 on caprine granulosa cell steroidogenesis, in vitro" @default.
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- W1973219123 doi "https://doi.org/10.1016/s0921-4488(99)00020-6" @default.
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