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• W1973250838 endingPage "1112" @default.
• W1973250838 startingPage "1107" @default.
• W1973250838 abstract "In situ padlock/rolling circle amplification (RCA) is a method used to amplify, visualize, and quantify target DNA molecules in cells. However, the multiple reaction steps involved make this technique costly and cumbersome. We developed a novel, simplified, automated microfluidic system for RCA, and demonstrated its effectiveness by counting amplified mitochondrial DNA fragments in HeLa cells. After optimizing the volume of the reaction solutions and washing buffer composition, the product yield was equal to that obtained by the conventional manual method. The required volume of reagents was reduced to 10 µL, which is less than half the volume used in the conventional method. To the best of our knowledge, this is the first report of an automated microfluidic method for in situ padlock/RCA, which can be useful for making highly efficient pathological diagnoses." @default.
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• W1973250838 date "2014-12-01" @default.
• W1973250838 modified "2023-09-26" @default.
• W1973250838 title "Microfluidics-based in situ Padlock/Rolling Circle Amplification System for Counting Single DNA Molecules in a Cell" @default.
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• W1973250838 doi "https://doi.org/10.2116/analsci.30.1107" @default.
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