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- W1973277730 abstract "Abstract The usefulness of five extenders for cryopreservation of muskellunge semen was studied in fertilization trials and computer-assisted semen analysis (CASA) of postthaw sperm motility. The effect of prefreezing storage time before cryopreservation on success of cryopreservation was also analyzed. A 50% fertilization rate (compared with fresh semen) of the extender containing 10% dimethyl sulfoxide (DMSO) in seminal-plasma-mimicking medium (SPMM) supplemented by 10% egg yolk was significantly higher than in extenders consisting of 10% DMSO, 0.6 M sucrose, and 10% egg yolk or 20% glycerol and 0.3 M glucose. Spermatozoa cryopreserved with DMSO–SPMM–egg yolk extender had the highest values of straight-line velocity and linearity of movement. Significantly lower fertilizing ability and curvilinear velocity were found for semen specimens stored on ice for 5 h before freezing compared with specimens stored for 1 h. Although spermatozoa cryopreserved with extender containing 10% methanol had only traces of..." @default.
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- W1973277730 date "1999-05-01" @default.
- W1973277730 modified "2023-09-22" @default.
- W1973277730 title "Effects of Extenders and Time of Storage before Freezing on Motility and Fertilization of Cryopreserved Muskellunge Spermatozoa" @default.
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- W1973277730 doi "https://doi.org/10.1577/1548-8659(1999)128<0542:eoeato>2.0.co;2" @default.
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