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- W1973278712 abstract "Heavy-metal ions are commonly used as staining agents. An important property in staining with metal ions is that the resulting tissue–metal complex be sufficiently stable to withstand subsequent manipulations without reversible dissociation. This stability may be either thermodynamic (that is, the complex has a sufficiently high formation constant) or kinetic (that is, the rate of dissociation is very slow as compared to the time for tissue manipulation). Where metal chelates are sufficiently stable in either of these senses, they offer the advantage of a clean staining because better washing procedures can be used and nonspecific deposition is diminished. One type of metal-containing stain relies on the simple precipitation of inorganic species, usually because of enzyme activity on an appropriate substrate. Thus, phosphatases can be visualized by the liberation of phosphate, with subsequent precipitation of lead phosphate following the addition of lead ions. One type of method commonly used for electron staining of tissues with heavy-metal complexes depends on the adjustment of conditions so that the metal complex in the solution is near the limit of its stability. Immersion of tissue sections in the solution allows binding groups on the tissue to compete successfully for some of the metal ion present and hence, to undergo staining." @default.
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- W1973278712 date "1969-11-01" @default.
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- W1973278712 title "Alcian blue as an electron stain" @default.
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- W1973278712 doi "https://doi.org/10.1016/0014-4827(69)90131-1" @default.
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