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- W1973282004 abstract "An optical method is presented that allows the measurement of the triplet lifetime of a fluorescent molecule. This is a characteristic specific to each fluorophore. Based on differences in triplet lifetimes of two fluorescent species (autofluorescence versus label), this novel approach measures relative quantities of a transmembrane receptor and associated fluorescently labeled ligand during its recycling in living cells. Similarly to fluorescence-lifetime based methods, our approach is almost insensitive to photobleaching. A simple theory for unmixing two known triplet lifetimes is presented along with validation of the method by measurements of transferrin recycling in a model system based on chinese hamster ovarian cells (CHO). Transferrin is the delivery carrier for Fe(3+) to the cell." @default.
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- W1973282004 date "2012-09-13" @default.
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- W1973282004 title "Assessment of transferrin recycling by Triplet Lifetime Imaging in living cells" @default.
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- W1973282004 doi "https://doi.org/10.1364/boe.3.002526" @default.
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