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- W1973282362 abstract "A new satellite-like replicating RNA found in association with cucumber mosaic virus type 1 (CMV-1), one of the earliest CMV isolates described (Doolittle, 1920), was identified and partially characterized. CMV-1-associated RNA 5, or (1)CARNA 5, was isolated from CMV-1 propagated in tomato, where it failed to induce lethal necrosis. In this way it was distinguished from CARNA 5 with the tomato necrosis-causing ability, which was redesignated (n)CARNA 5. Although (1)CARNA 5 and (n)CARNA 5 could be separated by 7.5% polyacrylamide gel electrophoresis under certain conditions, the molecules are probably identical in size, or nearly so. (1)CARNA 5 and (n)CARNA 5 have only limited nucleotide sequence homology. This is also evident from the different multitransitional absorbance-temperature profiles that can be obtained during the thermal denaturation of their respective double-stranded forms. In mixed infections in tomato, the replication of (1)CARNA 5 was also supported by CMV strains WT or S, while (n)CARNA 5 was supported by CMV-1. The biologically different (1)CARNA 5 and (n)CARNA 5 can be considered sequence variants among a group of small satellite-like RNA molecules dependent upon CMV for their replication." @default.
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- W1973282362 date "1981-10-01" @default.
- W1973282362 modified "2023-09-22" @default.
- W1973282362 title "Cucumber nosaic virus-associated RNA 5 VIII. Identification and partial characterization of a CARNA 5 incapable of inducing tomato necrosis" @default.
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- W1973282362 doi "https://doi.org/10.1016/0042-6822(81)90232-4" @default.
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