FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1973284416> ?p ?o ?g. }

• W1973284416 endingPage "615" @default.
• W1973284416 startingPage "607" @default.
• W1973284416 abstract "Background Induction of apoptosis by tamoxifen has been postulated to involve oxidative stress. Tamoxifen (TAM) may act on estrogen receptors (ER) located in the plasma membrane. Our hypothesis that supplemental antioxidant vitamin E (α-tocopherol) acts at the plasma membrane to alter the effectiveness of tamoxifen was tested in ER-positive breast cancer cell lines, MCF-7 and T47D. Methods Cells were treated in vitro with 20-μM TAM alone and in combination with 10-μM α-tocopherol (AT). Estrogen growth signals were quantified by immunohistochemical staining for the mitogen-activated protein kinase p-ERK. Rapid changes in intracellular calcium were detected in TAM-treated MCF-7 and T-47D cells by fluorescence microscopy of cells loaded with the calcium-sensitive dye Fluo 4AM. Apoptosis was assayed by flow cytometry. Results Proliferating cells in normal medium exhibited strong p-ERK staining. Addition of TAM abolished p-ERK staining and caused cell rounding and death. The addition of AT led to the restoration of cell proliferation and p-ERK expression even in the presence of high-dose TAM. Intracellular calcium rapidly increased in MCF-7 and T47D cells upon exposure to TAM, followed by an increase in caspase activation and eventual apoptosis. The increase in intracellular calcium was abolished by the addition of 10μM AT to TAM, and pan-caspase staining decreased at 5 hours from 72% to 41%. Conclusions These studies suggest that supplemental vitamin E decreases the inhibitory effect of TAM on the proliferation of ER+ breast cancer cells and eliminates the rapid rise in intracellular calcium that leads to apoptosis stimulated by TAM. The use of vitamin E acetate supplements may be inadvisable for women taking tamoxifen. Induction of apoptosis by tamoxifen has been postulated to involve oxidative stress. Tamoxifen (TAM) may act on estrogen receptors (ER) located in the plasma membrane. Our hypothesis that supplemental antioxidant vitamin E (α-tocopherol) acts at the plasma membrane to alter the effectiveness of tamoxifen was tested in ER-positive breast cancer cell lines, MCF-7 and T47D. Cells were treated in vitro with 20-μM TAM alone and in combination with 10-μM α-tocopherol (AT). Estrogen growth signals were quantified by immunohistochemical staining for the mitogen-activated protein kinase p-ERK. Rapid changes in intracellular calcium were detected in TAM-treated MCF-7 and T-47D cells by fluorescence microscopy of cells loaded with the calcium-sensitive dye Fluo 4AM. Apoptosis was assayed by flow cytometry. Proliferating cells in normal medium exhibited strong p-ERK staining. Addition of TAM abolished p-ERK staining and caused cell rounding and death. The addition of AT led to the restoration of cell proliferation and p-ERK expression even in the presence of high-dose TAM. Intracellular calcium rapidly increased in MCF-7 and T47D cells upon exposure to TAM, followed by an increase in caspase activation and eventual apoptosis. The increase in intracellular calcium was abolished by the addition of 10μM AT to TAM, and pan-caspase staining decreased at 5 hours from 72% to 41%. These studies suggest that supplemental vitamin E decreases the inhibitory effect of TAM on the proliferation of ER+ breast cancer cells and eliminates the rapid rise in intracellular calcium that leads to apoptosis stimulated by TAM. The use of vitamin E acetate supplements may be inadvisable for women taking tamoxifen." @default.
• W1973284416 created "2016-06-24" @default.
• W1973284416 creator A5002516215 @default.
• W1973284416 creator A5010701544 @default.
• W1973284416 creator A5034990957 @default.
• W1973284416 creator A5057227829 @default.
• W1973284416 creator A5090540221 @default.
• W1973284416 date "2006-10-01" @default.
• W1973284416 modified "2023-09-26" @default.
• W1973284416 title "Effect of vitamin E on tamoxifen-treated breast cancer cells" @default.
• W1973284416 cites W1566775766 @default.
• W1973284416 cites W1662062786 @default.
• W1973284416 cites W1935573162 @default.
• W1973284416 cites W1940935006 @default.
• W1973284416 cites W1975221601 @default.
• W1973284416 cites W1981161903 @default.
• W1973284416 cites W1999014427 @default.
• W1973284416 cites W2001399393 @default.
• W1973284416 cites W2016569614 @default.
• W1973284416 cites W2020462357 @default.
• W1973284416 cites W2023384375 @default.
• W1973284416 cites W2049116118 @default.
• W1973284416 cites W2069916372 @default.
• W1973284416 cites W2085635715 @default.
• W1973284416 cites W2086383194 @default.
• W1973284416 cites W2098956709 @default.
• W1973284416 cites W2099604555 @default.
• W1973284416 cites W2100914972 @default.
• W1973284416 cites W2107186334 @default.
• W1973284416 cites W2111973875 @default.
• W1973284416 cites W2113388598 @default.
• W1973284416 cites W2122015809 @default.
• W1973284416 cites W2127681400 @default.
• W1973284416 cites W2128069055 @default.
• W1973284416 cites W2137355132 @default.
• W1973284416 cites W2141232624 @default.
• W1973284416 cites W2164551904 @default.
• W1973284416 cites W2166631738 @default.
• W1973284416 cites W2901368272 @default.
• W1973284416 cites W4236030394 @default.
• W1973284416 cites W4249334806 @default.
• W1973284416 cites W4319718429 @default.
• W1973284416 cites W91452638 @default.
• W1973284416 doi "https://doi.org/10.1016/j.surg.2006.07.007" @default.
• W1973284416 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17011908" @default.
• W1973284416 hasPublicationYear "2006" @default.
• W1973284416 type Work @default.
• W1973284416 sameAs 1973284416 @default.
• W1973284416 citedByCount "31" @default.
• W1973284416 countsByYear W19732844162012 @default.
• W1973284416 countsByYear W19732844162013 @default.
• W1973284416 countsByYear W19732844162014 @default.
• W1973284416 countsByYear W19732844162015 @default.
• W1973284416 countsByYear W19732844162016 @default.
• W1973284416 countsByYear W19732844162017 @default.
• W1973284416 countsByYear W19732844162018 @default.
• W1973284416 countsByYear W19732844162019 @default.
• W1973284416 countsByYear W19732844162020 @default.
• W1973284416 countsByYear W19732844162022 @default.
• W1973284416 countsByYear W19732844162023 @default.
• W1973284416 crossrefType "journal-article" @default.
• W1973284416 hasAuthorship W1973284416A5002516215 @default.
• W1973284416 hasAuthorship W1973284416A5010701544 @default.
• W1973284416 hasAuthorship W1973284416A5034990957 @default.
• W1973284416 hasAuthorship W1973284416A5057227829 @default.
• W1973284416 hasAuthorship W1973284416A5090540221 @default.
• W1973284416 hasConcept C121608353 @default.
• W1973284416 hasConcept C126322002 @default.
• W1973284416 hasConcept C134018914 @default.
• W1973284416 hasConcept C181080969 @default.
• W1973284416 hasConcept C184235292 @default.
• W1973284416 hasConcept C190283241 @default.
• W1973284416 hasConcept C2777176818 @default.
• W1973284416 hasConcept C31573885 @default.
• W1973284416 hasConcept C502942594 @default.
• W1973284416 hasConcept C519063684 @default.
• W1973284416 hasConcept C530470458 @default.
• W1973284416 hasConcept C55493867 @default.
• W1973284416 hasConcept C57074206 @default.
• W1973284416 hasConcept C62112901 @default.
• W1973284416 hasConcept C71924100 @default.
• W1973284416 hasConcept C79879829 @default.
• W1973284416 hasConcept C84606932 @default.
• W1973284416 hasConcept C86803240 @default.
• W1973284416 hasConcept C95444343 @default.
• W1973284416 hasConceptScore W1973284416C121608353 @default.
• W1973284416 hasConceptScore W1973284416C126322002 @default.
• W1973284416 hasConceptScore W1973284416C134018914 @default.
• W1973284416 hasConceptScore W1973284416C181080969 @default.
• W1973284416 hasConceptScore W1973284416C184235292 @default.
• W1973284416 hasConceptScore W1973284416C190283241 @default.
• W1973284416 hasConceptScore W1973284416C2777176818 @default.
• W1973284416 hasConceptScore W1973284416C31573885 @default.
• W1973284416 hasConceptScore W1973284416C502942594 @default.
• W1973284416 hasConceptScore W1973284416C519063684 @default.
• W1973284416 hasConceptScore W1973284416C530470458 @default.
• W1973284416 hasConceptScore W1973284416C55493867 @default.
• W1973284416 hasConceptScore W1973284416C57074206 @default.

• next