FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1973308380> ?p ?o ?g. }

• W1973308380 endingPage "64" @default.
• W1973308380 startingPage "55" @default.
• W1973308380 abstract "Real-time PCR represents a new methodology that accurately quantifies nucleic acids. This has been made possible by the use of fluorogenic probes, which are presented in two forms, namely hydrolysis probes (also called TaqMan probes) and hybridisation probes. We decided to apply this methodology to cytokine mRNA quantification and this led us to the development of a protocol that provides an easy way to develop and perform rapidly real-time PCR on a Lightcycler instrument. It was made possible by the use of freely available software that permits a choice of both the hydrolysis probe and the primers. We firstly demonstrated that the reproducibility of the method using hydrolysis probes compares favourably with that obtained with hybridisation probes. We then applied this technique to determine the kinetics of IL-1ra, IL-1β, IL-5, IL-13, TNF-α and IFN-γ induction upon stimulation of human peripheral blood mononuclear cells (PBMC) by phytohaemagglutinin (PHA). Finally, the method was also used successfully to demonstrate that IFN-α induces IL-10 mRNA accumulation in human monocytes." @default.
• W1973308380 created "2016-06-24" @default.
• W1973308380 creator A5004002800 @default.
• W1973308380 creator A5016249576 @default.
• W1973308380 creator A5025003426 @default.
• W1973308380 creator A5030002732 @default.
• W1973308380 creator A5048936767 @default.
• W1973308380 creator A5066073347 @default.
• W1973308380 creator A5075613865 @default.
• W1973308380 creator A5076384990 @default.
• W1973308380 date "2002-01-01" @default.
• W1973308380 modified "2023-10-18" @default.
• W1973308380 title "Cytokine mRNA quantification by real-time PCR" @default.
• W1973308380 cites W1569947224 @default.
• W1973308380 cites W1578914995 @default.
• W1973308380 cites W1690462967 @default.
• W1973308380 cites W183054515 @default.
• W1973308380 cites W1965472353 @default.
• W1973308380 cites W1972985557 @default.
• W1973308380 cites W1994091239 @default.
• W1973308380 cites W2022333173 @default.
• W1973308380 cites W2026318747 @default.
• W1973308380 cites W2030348506 @default.
• W1973308380 cites W2051804704 @default.
• W1973308380 cites W2062143298 @default.
• W1973308380 cites W2070721758 @default.
• W1973308380 cites W2093901599 @default.
• W1973308380 cites W2098029146 @default.
• W1973308380 cites W2109971798 @default.
• W1973308380 cites W2113001043 @default.
• W1973308380 cites W2164578725 @default.
• W1973308380 cites W4254533614 @default.
• W1973308380 cites W4313333765 @default.
• W1973308380 doi "https://doi.org/10.1016/s0022-1759(01)00489-6" @default.
• W1973308380 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11730841" @default.
• W1973308380 hasPublicationYear "2002" @default.
• W1973308380 type Work @default.
• W1973308380 sameAs 1973308380 @default.
• W1973308380 citedByCount "200" @default.
• W1973308380 countsByYear W19733083802012 @default.
• W1973308380 countsByYear W19733083802013 @default.
• W1973308380 countsByYear W19733083802014 @default.
• W1973308380 countsByYear W19733083802015 @default.
• W1973308380 countsByYear W19733083802016 @default.
• W1973308380 countsByYear W19733083802017 @default.
• W1973308380 countsByYear W19733083802018 @default.
• W1973308380 countsByYear W19733083802019 @default.
• W1973308380 countsByYear W19733083802020 @default.
• W1973308380 countsByYear W19733083802021 @default.
• W1973308380 countsByYear W19733083802022 @default.
• W1973308380 countsByYear W19733083802023 @default.
• W1973308380 crossrefType "journal-article" @default.
• W1973308380 hasAuthorship W1973308380A5004002800 @default.
• W1973308380 hasAuthorship W1973308380A5016249576 @default.
• W1973308380 hasAuthorship W1973308380A5025003426 @default.
• W1973308380 hasAuthorship W1973308380A5030002732 @default.
• W1973308380 hasAuthorship W1973308380A5048936767 @default.
• W1973308380 hasAuthorship W1973308380A5066073347 @default.
• W1973308380 hasAuthorship W1973308380A5075613865 @default.
• W1973308380 hasAuthorship W1973308380A5076384990 @default.
• W1973308380 hasConcept C104317684 @default.
• W1973308380 hasConcept C105580179 @default.
• W1973308380 hasConcept C137061746 @default.
• W1973308380 hasConcept C143660080 @default.
• W1973308380 hasConcept C153911025 @default.
• W1973308380 hasConcept C185592680 @default.
• W1973308380 hasConcept C202751555 @default.
• W1973308380 hasConcept C203014093 @default.
• W1973308380 hasConcept C24107716 @default.
• W1973308380 hasConcept C2778690821 @default.
• W1973308380 hasConcept C2780050583 @default.
• W1973308380 hasConcept C3017910909 @default.
• W1973308380 hasConcept C43617362 @default.
• W1973308380 hasConcept C48023723 @default.
• W1973308380 hasConcept C55493867 @default.
• W1973308380 hasConcept C70721500 @default.
• W1973308380 hasConcept C86803240 @default.
• W1973308380 hasConcept C9893847 @default.
• W1973308380 hasConceptScore W1973308380C104317684 @default.
• W1973308380 hasConceptScore W1973308380C105580179 @default.
• W1973308380 hasConceptScore W1973308380C137061746 @default.
• W1973308380 hasConceptScore W1973308380C143660080 @default.
• W1973308380 hasConceptScore W1973308380C153911025 @default.
• W1973308380 hasConceptScore W1973308380C185592680 @default.
• W1973308380 hasConceptScore W1973308380C202751555 @default.
• W1973308380 hasConceptScore W1973308380C203014093 @default.
• W1973308380 hasConceptScore W1973308380C24107716 @default.
• W1973308380 hasConceptScore W1973308380C2778690821 @default.
• W1973308380 hasConceptScore W1973308380C2780050583 @default.
• W1973308380 hasConceptScore W1973308380C3017910909 @default.
• W1973308380 hasConceptScore W1973308380C43617362 @default.
• W1973308380 hasConceptScore W1973308380C48023723 @default.
• W1973308380 hasConceptScore W1973308380C55493867 @default.
• W1973308380 hasConceptScore W1973308380C70721500 @default.
• W1973308380 hasConceptScore W1973308380C86803240 @default.
• W1973308380 hasConceptScore W1973308380C9893847 @default.
• W1973308380 hasIssue "1-2" @default.
• W1973308380 hasLocation W19733083801 @default.

• next