Matches in SemOpenAlex for { <https://semopenalex.org/work/W1973321158> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W1973321158 endingPage "374" @default.
- W1973321158 startingPage "366" @default.
- W1973321158 abstract "A large decrease in the activity of two sequence-specific DNA-binding proteins implicated in transcription control was seen when these were affinity purified and assayed under standard conditions in electrophoretic mobility shift assays. Increasing the concentration of bovine serum albumin in the reaction mixtures from 0.1 to 5 mg/ml stimulated the DNA-binding activity of these affinity-purified proteins, human CREB (cyclic AMP response element binding protein) and MDBP (methylated DNA-binding protein), approximately 5-to more than 20-fold. In the case of affinity-purified MDBP, adding back the affinity flow-through fraction to the assay mixture gave similar extents of stimulation at much lower final protein concentrations. The specific DNA-binding activity of the affinity-purified CREB, but not that of MDBP, was also increased by adding a nonionic detergent to the binding reaction buffer although not as much. The large increase in the amount of MDBP · DNA complex seen upon supplementation of the affinity-purified MDBP with the affinity flow-through fraction or 5 mg/ml of BSA was shown to be due to stimulation, by nonspecific proteins, of specific complex formation and not to prevention of activity losses by adsorption or denaturation during the assay." @default.
- W1973321158 created "2016-06-24" @default.
- W1973321158 creator A5015332977 @default.
- W1973321158 creator A5041376137 @default.
- W1973321158 creator A5060693270 @default.
- W1973321158 creator A5085099623 @default.
- W1973321158 date "1992-03-01" @default.
- W1973321158 modified "2023-10-18" @default.
- W1973321158 title "Increasing the activity of affinity-purified DNA-binding proteins by adding high concentrations of nonspecific proteins" @default.
- W1973321158 cites W1506763963 @default.
- W1973321158 cites W1585759343 @default.
- W1973321158 cites W1601182054 @default.
- W1973321158 cites W1660661459 @default.
- W1973321158 cites W1670076014 @default.
- W1973321158 cites W1964351745 @default.
- W1973321158 cites W1987557547 @default.
- W1973321158 cites W1987717753 @default.
- W1973321158 cites W2003920409 @default.
- W1973321158 cites W2025066582 @default.
- W1973321158 cites W2028507406 @default.
- W1973321158 cites W2031205489 @default.
- W1973321158 cites W2041639550 @default.
- W1973321158 cites W2045677543 @default.
- W1973321158 cites W2064856877 @default.
- W1973321158 cites W2072403943 @default.
- W1973321158 cites W2080689622 @default.
- W1973321158 cites W2095566149 @default.
- W1973321158 cites W2111393771 @default.
- W1973321158 cites W2118757704 @default.
- W1973321158 cites W2153782423 @default.
- W1973321158 cites W2172270130 @default.
- W1973321158 cites W4247391250 @default.
- W1973321158 doi "https://doi.org/10.1016/0003-2697(92)90353-9" @default.
- W1973321158 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1385930" @default.
- W1973321158 hasPublicationYear "1992" @default.
- W1973321158 type Work @default.
- W1973321158 sameAs 1973321158 @default.
- W1973321158 citedByCount "21" @default.
- W1973321158 crossrefType "journal-article" @default.
- W1973321158 hasAuthorship W1973321158A5015332977 @default.
- W1973321158 hasAuthorship W1973321158A5041376137 @default.
- W1973321158 hasAuthorship W1973321158A5060693270 @default.
- W1973321158 hasAuthorship W1973321158A5085099623 @default.
- W1973321158 hasConcept C104317684 @default.
- W1973321158 hasConcept C13965031 @default.
- W1973321158 hasConcept C179247698 @default.
- W1973321158 hasConcept C181199279 @default.
- W1973321158 hasConcept C183978625 @default.
- W1973321158 hasConcept C185592680 @default.
- W1973321158 hasConcept C198684833 @default.
- W1973321158 hasConcept C2776923230 @default.
- W1973321158 hasConcept C43617362 @default.
- W1973321158 hasConcept C552990157 @default.
- W1973321158 hasConcept C55493867 @default.
- W1973321158 hasConcept C86339819 @default.
- W1973321158 hasConcept C86807702 @default.
- W1973321158 hasConcept C94966510 @default.
- W1973321158 hasConceptScore W1973321158C104317684 @default.
- W1973321158 hasConceptScore W1973321158C13965031 @default.
- W1973321158 hasConceptScore W1973321158C179247698 @default.
- W1973321158 hasConceptScore W1973321158C181199279 @default.
- W1973321158 hasConceptScore W1973321158C183978625 @default.
- W1973321158 hasConceptScore W1973321158C185592680 @default.
- W1973321158 hasConceptScore W1973321158C198684833 @default.
- W1973321158 hasConceptScore W1973321158C2776923230 @default.
- W1973321158 hasConceptScore W1973321158C43617362 @default.
- W1973321158 hasConceptScore W1973321158C552990157 @default.
- W1973321158 hasConceptScore W1973321158C55493867 @default.
- W1973321158 hasConceptScore W1973321158C86339819 @default.
- W1973321158 hasConceptScore W1973321158C86807702 @default.
- W1973321158 hasConceptScore W1973321158C94966510 @default.
- W1973321158 hasIssue "2" @default.
- W1973321158 hasLocation W19733211581 @default.
- W1973321158 hasLocation W19733211582 @default.
- W1973321158 hasOpenAccess W1973321158 @default.
- W1973321158 hasPrimaryLocation W19733211581 @default.
- W1973321158 hasRelatedWork W111381880 @default.
- W1973321158 hasRelatedWork W171189429 @default.
- W1973321158 hasRelatedWork W2000251996 @default.
- W1973321158 hasRelatedWork W2057013238 @default.
- W1973321158 hasRelatedWork W2088386724 @default.
- W1973321158 hasRelatedWork W2104121376 @default.
- W1973321158 hasRelatedWork W2143732305 @default.
- W1973321158 hasRelatedWork W2361099097 @default.
- W1973321158 hasRelatedWork W2468848913 @default.
- W1973321158 hasRelatedWork W4250447984 @default.
- W1973321158 hasVolume "201" @default.
- W1973321158 isParatext "false" @default.
- W1973321158 isRetracted "false" @default.
- W1973321158 magId "1973321158" @default.
- W1973321158 workType "article" @default.