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- W1973402824 abstract "Several cases of human infection caused by verotoxin-producing Escherichia coli (VTEC) O157:H7 in Sweden have been connected with cattle farm visits. Between 1996 and 2002, 18 farms were classified as the source of human cases with isolation of EHEC (Enterohaemorrhagic Escherichia coli) after VTEC O157:H7 had been isolated from cattle on those farms.Characterization by phage typing and molecular methods of the strains isolated from these 18 farms, including PCR for virulence genes (vtx1, vtx2 and variants thereof, eaeA and EHEC-hlyA) and Pulsed-Field Gel Electrophoresis (PFGE), demonstrated a cluster of very similar strains from 16 farms. All were of phage type 4, carried the genes encoding the verotoxins VT2 and VT2c, intimin, EHEC-haemolysin and flagellin H7 as shown by PCR, and had identical or very similar PFGE patterns. When analysing strains in a prevalence study of VTEC O157:H7 from cattle at slaughter as well as from an on-farm prevalence study of dairy cattle, using the same typing methods, a rather wide variation was observed among the isolated VTEC O157:H7 strains.In Sweden, a limited group of genetically similar and highly pathogenic VTEC O157:H7 strains seem to predominate in direct or indirect transmission from cattle to man." @default.
- W1973402824 created "2016-06-24" @default.
- W1973402824 creator A5015492815 @default.
- W1973402824 creator A5051590156 @default.
- W1973402824 date "2010-01-01" @default.
- W1973402824 modified "2023-09-23" @default.
- W1973402824 title "Verotoxigenic Escherichia coli O157:H7 from Swedish cattle; isolates from prevalence studies versus strains linked to human infections - A retrospective study" @default.
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- W1973402824 doi "https://doi.org/10.1186/1746-6148-6-7" @default.
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